Nocardioides currus sp nov., isolated from a mobile car air-conditioning system

A strictly aerobic Gram-stain-positive bacterial strain, designated IB-3(T), was isolated from a car air-conditioning system in the Republic of Korea. Cells were non-motile rods showing catalase- and oxidase-positive reactions. Growth of IB-3(T) was observed at 20-40 degrees C (optimum, 25 degrees C), at pH 6.5-9.0 (optimum, pH 7.5) and in the presence of 0-1% (w/v) NaCl (optimum, 0%). Menaquinone-8 (H-4) was detected as the predominant respiratory quinone and iso-C-16:0, 10-methyl-C-17:0, iso-C-17:0, C-18:19c, C-17:18c, C-18:0, 10-methyl-C-18:0 (TBSA) and C-17:0 were identified as the major cellular fatty acids. Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and phosphatidylinositol were detected as the major polar lipids. The major cell wall peptidoglycan type was ll-2,6-diaminopimelic acid. The G+C content of the genomic DNA was 71.5mol%. IB-3(T) was most closely related to Nocardioides terrigenaDS-17T with a 98.0% 16S rRNA gene sequence similarity. The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that IB-3T formed a distinct phylogenetic lineage within the genus Nocardioidesof the family Nocardioidaceae. On the basis of the phenotypic, chemotaxonomic and molecular features, IB-3T represents a novel species of the genus Nocardioides, for which the name Nocardioidescurrus sp. nov. is proposed. The type strain is IB-3(T) (=KACC 19522(T)=JCM 32672(T)).