circ_0001730 promotes proliferation and invasion via the miR-326/Wnt7B axis in glioma cells

被引：48
|
作者：
Lu, Yaoyong [1 ]
Deng, Xubin [2 ]
Xiao, Guanghui [3 ]
Zheng, Xin [4 ]
Ma, Lei [2 ]
Huang, Wendong [5 ]
机构：
[1] Gaozhou Peoples Hosp, Sect 3, Dept Oncol, Gaozhou, Guangdong, Peoples R China
[2] Guangzhou Med Univ, Affiliated Canc Hosp & Inst, Dept Internal Med, Guangzhou, Guangdong, Peoples R China
[3] Fox Chase Canc Ctr, Dept Canc Biol Program, Canc Biol Program, Philadelphia, PA 215 USA
[4] Southern Med Univ, Yanling Hosp, Dept Internal Med, Guangzhou, Guangdong, Peoples R China
[5] Maoming Peoples Hosp, Dept Pharm, Maoming, Guangdong, Peoples R China
来源：
EPIGENOMICS | 2019年 / 11卷 / 11期
关键词：
beta-catenin; circRNAs; epithelial to mesenchymal transition; glioblastoma; miR-326; Wnt7B; CIRCULAR RNA; BIOLOGICAL BEHAVIORS; CANCER; EXPRESSION; WNT7B; ADULTS; BRAIN; MIR-7;
D O I：
10.2217/epi-2019-0121
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Aim: To study the role of circRNA (circ_0001730) in glioblastoma. Materials & methods: The interaction between circ 0001730 and miR-326 was confirmed by FISH, RNA pull down, RNA-binding protein immunoprecipitation and luciferase reporter assays. Cell proliferation and growth were determined by MTT, EdU and colony formation assays. Cell migration was assessed by the Boyden assay. Results: The levels of circ_0001730 were elevated in glioblastoma cell lines and tissues. circ_0001730 downregulation suppressed migration and proliferation in glioblastoma cells. SP1 bounds to the promoter of circ 0001730 host gene EPHB4 thereby increasing the expression of circ_0001730. circ_0001730 activated the Wnt/beta-catenin pathway via the miR-326/Wnt7B axis. Conclusion: circ_000173 promoted growth and invasion in glioblastoma cells via the miR-326/Wnt7B axis.
引用
收藏
页码：1335 / 1353
页数：19
相关论文
共 50 条
  • [21] Circular RNA hsa_circ_0005397 promotes hepatocellular carcinoma progression by regulating the miR-326/PDK2 axis
    Gong, Jianzhuang
    Du, Chenxu
    Sun, Nai
    Xiao, Xingguo
    Wu, Huili
    JOURNAL OF GENE MEDICINE, 2021, 23 (06):
  • [22] LncRNA SNHG3 Promotes Gastric Cancer Cells Proliferation, Migration, and Invasion by Targeting miR-326 (vol 2021, 9935410, 2021)
    Rao, Jun
    Fu, Jinjin
    Meng, Chuchen
    Huang, Jin
    Qin, Xiangrong
    Zhuang, Shaohua
    JOURNAL OF ONCOLOGY, 2022, 2022
  • [23] Adam17, a Target of Mir-326, Promotes Emt-Induced Cells Invasion in Lung Adenocarcinoma
    Cai, Ming
    Wang, Zhiqiang
    Zhang, Jiru
    Zhou, Huan
    Jin, Linfang
    Bai, Ruizhen
    Weng, Yuan
    CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2015, 36 (03) : 1175 - 1185
  • [24] LncRNA OIP5-AS1/miR-410-3p/Wnt7b axis promotes the proliferation of rheumatoid arthritis fibroblast-like synoviocytes via regulating the Wnt/β-catenin pathway
    Sun, Yuan
    Jiang, Hui
    Pan, LingYu
    Han, YanQuan
    Chen, Yan
    Jiang, Yeke
    Wang, Yongzhong
    AUTOIMMUNITY, 2023, 56 (01)
  • [25] circ-PSD3 promoted proliferation and invasion of papillary thyroid cancer cells via regulating the miR-7-5p/METTL7B axis
    Zhu, Jialun
    Wang, Yongbin
    Yang, Chuanzhou
    Feng, Zhiping
    Huang, Yanni
    Liu, Pengjie
    Chen, Fukun
    Deng, Zhiyong
    JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION, 2022, 42 (03) : 251 - 260
  • [26] Hsa_circ_0005075 promotes the proliferation, migration, and invasion of colorectal cancer cells via miR-431/DDX5 axis
    Luo, J.
    Li, B.
    Liu, Y.
    Feng, H. Y.
    JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS, 2021, 35 (06): : 1847 - 1860
  • [27] Circular RNA circ_0000517 regulates hepatocellular carcinoma development via miR-326/IGF1R axis
    He, Shuwei
    Yang, Jianzeng
    Jiang, Shitao
    Li, Yuan
    Han, Xingmin
    CANCER CELL INTERNATIONAL, 2020, 20 (01)
  • [28] Circular RNA hsa_circ_0000517 modulates hepatocellular carcinoma advancement via the miR-326/SMAD6 axis
    He, Shuwei
    Guo, Zhengwu
    Kang, Qian
    Wang, Xu
    Han, Xingmin
    CANCER CELL INTERNATIONAL, 2020, 20 (01)
  • [29] lncRNA RPPH1 promotes non-small cell lung cancer progression through the miR-326/WNT2B axis
    Wu, Yuying
    Cheng, Kewei
    Liang, Wenjun
    Wang, Xiaohua
    ONCOLOGY LETTERS, 2020, 20 (04)
  • [30] Circular RNA hsa_circ_0003892 promotes the development of papillary thyroid carcinoma by regulating the miR-326/LASP1 axis
    Han, Peng
    Liu, Junsong
    Zhao, Qian
    Li, Honghui
    Zhang, Ting
    Li, Baiya
    Niu, Xiaorong
    HISTOLOGY AND HISTOPATHOLOGY, 2023, 38 (05) : 585 - 595
12345