Current status and outlook of advances in exosome isolation

被引：109

作者：

Liu, Wen-Zhao ^{[1
,2
]}

Ma, Zhan-Jun ^{[3
]}

Kang, Xue-Wen ^{[1
,2
,4
]}

机构：

[1] Lanzhou Univ, Clin Med Coll 2, Lanzhou 730030, Gansu, Peoples R China

[2] Lanzhou Univ, Dept Orthoped, Hosp 2, 82 Cuiyingmen St, Lanzhou 730030, Gansu, Peoples R China

[3] Catholic Univ Louvain, Louvain Drug Res Inst, UCLouvain, Adv Drug Delivery & Biomat, B-1200 Brussels, Belgium

[4] Int Cooperat Base Gansu Prov Pain Res Spinal Diso, Lanzhou 730000, Gansu, Peoples R China

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2022年 / 414卷 / 24期

关键词：

Exosome; Extracellular vesicle; Microfluidic; Isolation; Separation; FIELD-FLOW FRACTIONATION; EXTRACELLULAR VESICLES; WHOLE-BLOOD; SEPARATION; CAPTURE; PLASMA; SERUM; DIELECTROPHORESIS; NANOPARTICLES; BIOMARKERS;

D O I：

10.1007/s00216-022-04253-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract Exosomes are extracellular vesicles with a diameter ranging from 30 to 150 nm, which are an important medium for intercellular communication and are closely related to the progression of certain diseases. Therefore, exosomes are considered promising biomarkers for the diagnosis of specific diseases, and thereby, treatments based on exosomes are being widely examined. For exosome-related research, a rapid, simple, high-purity, and recovery isolation method is the primary prerequisite for exosomal large-scale application in medical practice. Although there are no standardized methods for exosome separation and analysis, various techniques have been established to explore their biochemical and physicochemical properties. In this review, we analyzed the progress in exosomal isolation strategies and proposed our views on the development prospects of various exosomal isolation techniques.

引用

页码：7123 / 7141

页数：19

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