The influence of different carbon sources on growth and single cell oil production in oleaginous yeasts Apiotrichum brassicae and Pichia kudriavzevii

被引:18
作者
Burgstaller, Lukas [1 ]
Loeffler, Sebastian [1 ]
De Marcellis, Luca [1 ]
Ghassemi, Khatereh [1 ]
Neureiter, Markus [1 ]
机构
[1] Univ Nat Resources & Life Sci, Inst Environm Biotechnol, Dept Agrobiotechnol IFA Tulln, Vienna BOKU, Vienna, Austria
基金
欧盟地平线“2020”;
关键词
Single cell oil; Apiotrichum brassicae; Pichia kudriavzevii; Volatile fatty acids; Oleaginous yeasts; VOLATILE FATTY-ACIDS; MICROBIAL LIPID PRODUCTION; CRYPTOCOCCUS-CURVATUS; RHODOSPORIDIUM-TORULOIDES; DENSITY CULTIVATION; FERMENTATION; CULTURE; GLYCEROL; BIOSYNTHESIS; ISSATCHENKIA;
D O I
10.1016/j.nbt.2022.02.003
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Oleaginous yeasts offer an interesting possibility for renewable lipid production, since the single cell oil accumulated can be based on a wide range of cheap, waste-derived carbon sources. Here, several short chain carboxylic acids and sugars commonly found in these substrates were assessed as carbon sources for Apiotrichum brassicae and Pichia kudriavzevii. While both strains were able to utilize all carbon sources employed, high volumetric lipid productivities (0.4 g/Lh) and lipid contents (68%) could be reached particularly with acetic acid as carbon source. Odd-numbered volatile fatty acids led to lower productivities and lipid contents, but the lipids contained unusually high proportions of odd-numbered fatty acids (up to 80% of total fatty acids). These fatty acids are rather uncommon in nature and might offer the possibility for various high value applications. In conclusion both strains are able to utilize a wide range of substrates potentially present in waste-derived substrates. Lipid content and volumetric lipid productivity strongly depend on the carbon source, with even numbered volatile fatty acids resulting in the highest values. For volatile fatty acids in particular, the carbon source also strongly influences the composition of the lipids produced by the yeast strains.
引用
收藏
页码:1 / 7
页数:7
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