Detection of Helicobacter pylori by Phenotypic and Genotypic Methods

被引：16
作者：
Fonseca, Tesie L. [1 ]
Moraes, Ernani P. [1 ]
Juliano, Carlos R. [1 ]
Silva, Ana M. [1 ]
Scaini, Carlos J. [1 ]
Mendoza-Sassi, Raul A. [1 ]
Silva, Pedro E. A. [1 ]
机构：
[1] Univ Fed Rio Grande, Rio Grande Do Sul, Brazil
来源：
DIGESTIVE DISEASES AND SCIENCES | 2010年 / 55卷 / 06期
关键词：
H; pylori; Diagnostic; cagA; ureA; POLYMERASE-CHAIN-REACTION; GASTRIC BIOPSY SPECIMENS; CAGA GENE; INFECTION; DIAGNOSIS; PCR; SUSCEPTIBILITY; CULTURE; REGION; DNA;
D O I：
10.1007/s10620-009-0928-8
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
This research evaluated the utilization of a urease in-house test, culture and molecular method (ureA PCR) as a diagnostic tool for Helicobacter pylori infection. Furthermore, we assessed the presence of the cagA gene in the specimens and in isolated strains that were positive for ureA by PCR positive. Sensitivity and specificity, respectively, were 100 and 95.8% for the urease in-house test 93.3 and 95.8 for the ureA PCR assay of the specimen and 100 and 100% for the culture. The presence of the cagA gene was observed in eight (53%) ureA-positive samples. In this study, we found that the PCR technique has applicability in the study of cagA, and other genes related to the H. pylori pathogen. This method can be applied to samples directly from biopsy or isolated from the bacteria.
引用
收藏
页码：1643 / 1648
页数：6
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