Serologic diagnosis of ovine lentiviruses by whole virus ELISA and AGID test

被引:15
作者
Celer, V
Celer, V
Nemcova, H
Zanoni, RG
Peterhans, E
机构
[1] Univ Vet & Pharmaceut Sci, Dept Immunol & Microbiol, CZ-61242 Brno, Czech Republic
[2] Univ Bern, Inst Vet Virol, Bern, Switzerland
来源
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH | 1998年 / 45卷 / 03期
关键词
D O I
10.1111/j.1439-0450.1998.tb00781.x
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion (AGID) are the most widely used serological tests for Maedi-Visna diagnostics. an indirect whole virus ELISA and an immunodiffusion test and compare their sensitivity. A total of 747 ovine serum specimens were analysed for antibodies against this ovine lentivirus. The number of positive results in the ELISA was 430 (57.56%). In the AGID test, a positive result was found in 380 samples (50.87%). In the group of discordant results 78 (10.4%) samples tested positive by the ELISA and negative by the AGID rest and 28 sera (3.7%) were found to be positive by the AGID test and negative by the ELISA. The data in this report show the ELISA to be more sensitive than the AGID rest, but accurate serological diagnostics should be based on a combination of the two tests.
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页码:183 / 188
页数:6
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