Selection of specific nanobodies to develop an immuno-assay detecting Staphylococcus aureus in milk

被引：53

作者：

Hu, Yaozhong ^{[1
]}

Sun, Ying ^{[2
]}

Gu, Jiaxin ^{[1
]}

Yang, Feier ^{[1
]}

Wu, Sihao ^{[1
]}

Zhang, Chuan ^{[2
]}

Ji, Xuemeng ^{[1
]}

Lv, Huan ^{[1
]}

Muyldermans, Serge ^{[3
]}

Wang, Shuo ^{[1
]}

机构：

[1] Nankai Univ, Sch Med, Tianjin Key Lab Food Sci & Hlth, Tianjin 300071, Peoples R China

[2] Tianjin Univ Sci Technol, Coll Food Sci & Engn, Tianjin 300457, Peoples R China

[3] Vrije Univ Brussel, Cellular & Mol Immunol, Brussels, Belgium

来源：

FOOD CHEMISTRY | 2021年 / 353卷

基金：

中国国家自然科学基金;

关键词：

Staphylococcus aureus; Food-borne pathogen; Nanobody; Sandwich ELISA; Immuno-assay; LISTERIA-MONOCYTOGENES; DOMAIN; ANTIBODIES; BINDING; PROTEIN; IDENTIFICATION; LIBRARY;

D O I：

10.1016/j.foodchem.2021.129481

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The interaction between conventional immunoglobulins (Igs) and the Ig-binding surface proteins of Staphylococcus aureus (S. aureus) have obstructed the development of immuno-assays to detect these bacteria. The current study aimed to select nanobodies (Nbs) recognizing specifically S. aureus and to establish an immuno-assay to uncover S. aureus contaminations in foods. An alpaca was immunized with an inactivated S. aureus strain followed by the construction of a Nb library from which four target-specific Nbs were retrieved. Subsequently, a sandwich ELISA employing the Nb147 and biotinylated-Nb147 pair to capture and to detect S. aureus, respectively, was established to possess a detection limit of 1.4 x 105 colony forming units (CFU)/mL. The dedicated immuno-assay has been verified by detecting 10 CFU/mL of S. aureus in milk samples after an 8 h-enrichment step. This study provides the basis of an easy, reproducible and effective immuno-assay to screen for S. aureus contaminations in foods.

引用

页数：10

共 40 条

[1] Evaluation of a nanobody phage display library constructed from a Brucella-immunised camel [J].

Abbady, A. Q. ;

Al-Mariri, A. ;

Zarkawi, M. ;

Al-Assad, A. ;

Muyldermans, S. .

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2011, 142 (1-2) :49-56

[2] S. aureus IgG-binding proteins SpA and Sbi:: Host speciticity and mechanisms of immune complex formation [J].

Atkins, Karen L. ;

Burman, Julia D. ;

Chamberlain, Emily S. ;

Cooper, Jessica E. ;

Poutrel, Bernard ;

Bagby, Stefan ;

Jenkins, A. Toby A. ;

Feil, Edward J. ;

van den Elsen, Jean M. H. .

MOLECULAR IMMUNOLOGY, 2008, 45 (06) :1600-1611

[3] Screening of Nanobody Specific for Peanut Major Allergen Ara h 3 by Phage Display [J].

Chen, Feng ;

Ma, Huan ;

Li, Yuelong ;

Wang, Heqiao ;

Samad, Abdus ;

Zhou, Jiahui ;

Zhu, Lixia ;

Zhang, Yuzhu ;

He, Jie ;

Fan, Xiaojiao ;

Jin, Tengchuan .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2019, 67 (40) :11219-11229

[4] Development of a Capture ELISA for Rapid Detection of Salmonella enterica in Food Samples [J].

Di Febo, Tiziana ;

Schirone, Maria ;

Visciano, Pierina ;

Portanti, Ottavio ;

Armillotta, Gisella ;

Persiani, Tiziana ;

Di Giannatale, Elisabetta ;

Tittarelli, Manuela ;

Luciani, Mirella .

FOOD ANALYTICAL METHODS, 2019, 12 (02) :322-330

[5]

Foster T. J, 2020, STAPHYLOCOCCUS AUREU

[6] NATURALLY-OCCURRING ANTIBODIES DEVOID OF LIGHT-CHAINS [J].

HAMERSCASTERMAN, C ;

ATARHOUCH, T ;

MUYLDERMANS, S ;

ROBINSON, G ;

HAMERS, C ;

SONGA, EB ;

BENDAHMAN, N ;

HAMERS, R .

NATURE, 1993, 363 (6428) :446-448

[7] Properties, production, and applications of camelid single-domain antibody fragments [J].

Harmsen, M. M. ;

De Haard, H. J. .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2007, 77 (01) :13-22

[8] Development of a specific nanobody and its application in rapid and selective determination of Salmonella enteritidis in milk [J].

He, Yixin ;

Ren, Yarong ;

Guo, Bing ;

Yang, Yifei ;

Ji, Yanwei ;

Zhang, Daohong ;

Wang, Jianlong ;

Wang, Yanru ;

Wang, Hong .

FOOD CHEMISTRY, 2020, 310

[9] Lectin-based detection of Escherichia coli and Staphylococcus aureus by flow cytometry [J].

Hendrickson, Olga D. ;

Nikitushkin, Vadim D. ;

Zherdev, Anatoly V. ;

Dzantiev, Boris B. .

ARCHIVES OF MICROBIOLOGY, 2019, 201 (03) :313-324

[10] S. aureus endocarditis: Clinical aspects and experimental approaches [J].

Hoerr, V ;

Franz, M. ;

Pletz, M. W. ;

Diab, M. ;

Niemann, S. ;

Faber, C. ;

Doenst, T. ;

Schulze, P. C. ;

Deinhardt-Emmer, S. ;

Loeffler, B. .

INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2018, 308 (06) :640-652

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