Metallo-β-lactamase-producing imipenem-resistant Pseudomonas aeruginosa clinical isolates in a university teaching hospital in Malaysia: detection of IMP-7 and first identification of IMP-4, VIM-2, and VIM-11

被引：15

作者：

Khosravi, Yalda ^{[1
]}

Tay, Sun Tee ^{[1
]}

Vadivelu, Jamuna ^{[1
]}

机构：

[1] Univ Malaya, Fac Med, Dept Med Microbiol, Kuala Lumpur 50603, Malaysia

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2010年 / 67卷 / 03期

关键词：

Metallo-beta-lactamase (MBL) genes; Pseudomonas aeruginosa; Imipenem resistance; bla(IMP); bla(VIM); Carbapenems; GENES; SPP;

D O I：

10.1016/j.diagmicrobio.2010.02.010

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Ninety (n = 90) imipenem-resistant Pseudomonas aeruginosa (IRPA) clinical isolates collected randomly during 2005 to 2008 from University Malaya Medical Center were assessed for the presence of different variants of metallo-beta-lactamase (MBL) genes. Polymerase chain reaction (PCR) assay detected 32 (n = 32) MBL gene PCR-positive isolates with the presence of bla(IMP) gene in 14 (n = 14) and bla(VIM) in 18 (n = 18) isolates. Four allelic variants, bla(IMP-7) (12 isolates), bla(IMP-4) (2 isolates), bla(VIM-2) (17 isolates), and bla(VIM-11) (1 isolate), of MBL genes were identified. This study is the first report of detection of bla(IMP-4), bla(VIM-2), and bla(VIM-11) MBL genes from IRPA clinical isolates in Malaysia. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：294 / 296

页数：3

共 19 条

[1]

[Anonymous], 2012, Performance Standards for Antimicrobial Disk Susceptibility Tests Approved Standard

[2] IMP-4-producing Pseudomonas aeruginosa in a French patient repatriated from Malaysia:: Impact of early detection and control measures [J].

Bert, Frederic ;

Vanjak, Dominique ;

Leflon-Guibout, Veronique ;

Mrejen, Serge ;

Delpierre, Sandrine ;

Redondo, Aimee ;

Nicolas-Chanoine, Marie-Helene .

CLINICAL INFECTIOUS DISEASES, 2007, 44 (05) :764-765

[3] Carbapenem Resistance among Pseudomonas aeruginosa Strains from India: Evidence for Nationwide Endemicity of Multiple Metallo-β-Lactamase Clones (VIM-2,-5,-6, and-11 and the Newly Characterized VIM-18) [J].

Castanheira, Mariana ;

Bell, Jan M. ;

Turnidge, John D. ;

Mathai, Dillip ;

Jones, Ronald N. .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (03) :1225-1227

[4] Nosocomial outbreak of carbapenem-resistant Pseudomonas aeruginosa with a new blaIMP allele, blaIMP-7 [J].

Gibb, AP ;

Tribuddharat, C ;

Moore, RA ;

Louie, TJ ;

Krulicki, W ;

Livermore, DM ;

Palepou, MFI .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (01) :255-258

[5] Carbapenem-resistant Pseudomonas aeruginosa in Malaysia producing IMP-7 β-lactamase [J].

Ho, SE ;

Subramaniam, G ;

Palasubramaniam, S ;

Navaratnam, P .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (10) :3286-3287

[6]

Hwa WE, 2009, J MICROBIOL IMMUNOL, V42, P54

[7] Pseudomonas aeruginosa: a formidable and ever-present adversary [J].

Kerr, K. G. ;

Snelling, A. M. .

JOURNAL OF HOSPITAL INFECTION, 2009, 73 (04) :338-344

[8]

Koh TH, 2008, ANN ACAD MED SINGAP, V37, P847

[9]

Kouda Shuntaro, 2007, Journal of Infection and Chemotherapy, V13, P276, DOI 10.1007/s10156-007-0520-0

[10] Evaluation of the Hodge test and the imipenem-EDTA double-disk synergy test for differentiating metallo-β-lactamase-producing isolates of Pseudomonas spp. and Acinetobacter spp. [J].

Lee, K ;

Lim, YS ;

Yong, D ;

Yum, JH ;

Chong, Y .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (10) :4623-4629

← 1 2 →