Non-viral delivery of an optogenetic tool into cells with self-healing hydrogel

被引：34

作者：

Hsieh, Fu-Yu ^{[1
]}

Han, Hao-Wei ^{[1
]}

Chen, Xiao-Ru ^{[2
]}

Yang, Chii-Shen ^{[2
]}

Wei, Yen ^{[3
]}

Hsu, Shan-hui ^{[1
,4
,5
]}

机构：

[1] Natl Taiwan Univ, Inst Polymer Sci & Engn, 1,Sec 4 Roosevelt Rd, Taipei 10617, Taiwan

[2] Natl Taiwan Univ, Dept Biochem Sci & Technol, 1,Sec 4 Roosevelt Rd, Taipei 10617, Taiwan

[3] Tsinghua Univ, Dept Chem, Minist Educ, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing, Peoples R China

[4] Natl Taiwan Univ, Ctr Tissue Engn & Printing 3D, Taipei, Taiwan

[5] Natl Hlth Res Inst, Inst Cellular & Syst Med, Zhunan, Taiwan

来源：

BIOMATERIALS | 2018年 / 174卷

关键词：

Self-healing hydrogel; Optogenetics; Bacteriorhodopsin; Neurodegenerative diseases; INTRACELLULAR PH; SYMPATHETIC NEURONS; OPTICAL CONTROL; DRIVEN; BRAIN; DIFFERENTIATION; SYSTEM; STIMULATION; RECOVERY; CHITOSAN;

D O I：

10.1016/j.biomaterials.2018.05.014

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Optogenetics offers unique, temporally precise control of neural activity in genetically targeted specific neurons that express light-sensitive opsin molecules. Three-dimensional (3D) delivery of optogenetics can be realized by co-injection of bacteriorhodopsin (HEBR) plasmid with a chitosan-based self-healing hydrogel with strong shear-thinning properties. The HEBR protein shows photoelectrical properties and can be used as an optical switch for cell activation. We optimize the shear force generated during the process of injection (similar to 100 Pa), which is transient because of the self-healing nature of the hydrogel. This transient force exerted by the self-healing hydrogel may allow the cytosolic delivery of HEBR plasmid with excellent cell viability and a high efficiency approaching 80%. When excited with green light, HEBR-delivered neural stem cells (NSCs) can proliferate and specifically differentiate into neurons in vitro and rescue the function of nerve impaired zebrafish in vivo. This novel optogenetic method combining 3D injectable self-healing hydrogel offers potential temporal-spatial approaches to treat neurodegenerative diseases in the future. (C) 2018 Elsevier Ltd. All rights reserved.

引用

页码：31 / 40

页数：10

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