Functional analysis of the stress response element and its role in the multistress response of Saccharomyces cerevisiae

被引:63
作者
Treger, JM [1 ]
Magee, TR [1 ]
McEntee, K [1 ]
机构
[1] Univ Calif Los Angeles, Sch Med, Dept Biol Chem, Ctr Hlth Sci 33 257, Los Angeles, CA 90095 USA
关键词
D O I
10.1006/bbrc.1997.8061
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The DDR2 gene of Saccharomyces cerevisiae is a multistress response gene whose transcription is rapidly and strongly induced by a diverse array of xenobiotic agents, and environmental and physiological conditions. The multistress response of this gene requires the pentanucleotide, 5'CCCCT, (C4T; STress Response Element)) and the zinc-finger transcription factors, Msn2p and Msn4p. A 51bp oligonucleotide (oligo 31/32) containing two STREs from the DDR2 promoter region was previously shown to direct heat shock activation of a lacZ reporter gene. In this work we demonstrate that the same element conferred a complete multistress response to an E. coli galK: reporter gene introduced into yeast cells. A variant oligonucleotide in which both the STRE spacing and neighboring sequences were altered responded to the same spectrum of stresses, while substitution of nucleotides within the pentanucleotide completely abolished the multistress response. These results directly demonstrate that STREs are not only necessary but are sufficient for mediating a transcriptional response to a surprisingly diverse set of environmental and physiological conditions. (C) 1998 Academic Press.
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页码:13 / 19
页数:7
相关论文
共 23 条
[1]   CONTROL OF SACCHAROMYCES-CEREVISIAE CATALASE T-GENE (CTT1) EXPRESSION BY NUTRIENT SUPPLY VIA THE RAS-CYCLIC AMP PATHWAY [J].
BISSINGER, PH ;
WIESER, R ;
HAMILTON, B ;
RUIS, H .
MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (03) :1309-1315
[2]   REGULATION OF THE YEAST METALLOTHIONEIN GENE [J].
GORMAN, JA ;
CLARK, PE ;
LEE, MC ;
DEBOUCK, C ;
ROSENBERG, M .
GENE, 1986, 48 (01) :13-22
[3]   YAP1P, A YEAST TRANSCRIPTIONAL ACTIVATOR THAT MEDIATES MULTIDRUG-RESISTANCE, REGULATES THE METABOLIC STRESS-RESPONSE [J].
GOUNALAKI, N ;
THIREOS, G .
EMBO JOURNAL, 1994, 13 (17) :4036-4041
[4]   THE DETERMINANTS OF HEAT-SHOCK ELEMENT-DIRECTED LACZ EXPRESSION IN SACCHAROMYCES-CEREVISIAE [J].
KIRK, N ;
PIPER, PW .
YEAST, 1991, 7 (06) :539-546
[5]   IDENTIFICATION OF CIS AND TRANS COMPONENTS OF A NOVEL HEAT-SHOCK STRESS REGULATORY PATHWAY IN SACCHAROMYCES-CEREVISIAE [J].
KOBAYASHI, N ;
MCENTEE, K .
MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (01) :248-256
[6]   THE DIVERGENTLY TRANSCRIBED GENES ENCODING YEAST RIBOSOMAL-PROTEINS L46 AND S24 ARE ACTIVATED BY SHARED RPG-BOXES [J].
KRAAKMAN, LS ;
MAGER, WH ;
MAURER, KTC ;
NIEUWINT, RTM ;
PLANTA, RJ .
NUCLEIC ACIDS RESEARCH, 1989, 17 (23) :9693-9706
[7]  
MAGER WH, 1993, BIOCHEM J, V290, P1
[8]   A SACCHAROMYCES-CEREVISIAE UAS ELEMENT CONTROLLED BY PROTEIN KINASE-A ACTIVATES TRANSCRIPTION IN RESPONSE TO A VARIETY OF STRESS CONDITIONS [J].
MARCHLER, G ;
SCHULLER, C ;
ADAM, G ;
RUIS, H .
EMBO JOURNAL, 1993, 12 (05) :1997-2003
[9]   The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress-response element (STRE) [J].
MartinezPastor, MT ;
Marchler, G ;
Schuller, C ;
MarchlerBauer, A ;
Ruis, H ;
Estruch, F .
EMBO JOURNAL, 1996, 15 (09) :2227-2235
[10]   SPECIFIC TRANSCRIPTS ARE ELEVATED IN SACCHAROMYCES-CEREVISIAE IN RESPONSE TO DNA DAMAGE [J].
MCCLANAHAN, T ;
MCENTEE, K .
MOLECULAR AND CELLULAR BIOLOGY, 1984, 4 (11) :2356-2363