Lophirones B and C Extenuate AFB1-Mediated Oxidative Onslaught on Cellular Proteins, Lipids, and DNA through Nrf-2 Expression

被引：21

作者：

Ajiboye, Taofeek O. ^{[1
]}

Yakubu, Musa T. ^{[2
]}

Oladiji, Adenike T. ^{[2
]}

机构：

[1] Al Hikmah Univ, Dept Biol Sci, Antioxidants Free Rad Funct Foods & Toxicol Res L, Ilorin, Nigeria

[2] Univ Ilorin, Dept Biochem, Phytomed Toxicol & Reprod Res Lab, Ilorin, Nigeria

来源：

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY | 2014年 / 28卷 / 12期

关键词：

Lophirones B and C; Nrf-2; Oxidative Stress Biomarkers; lipid Peroxidation; Aflatoxin B-1; AFLATOXIN B-1; RAT-LIVER; IN-VIVO; CHALCONE DIMERS; FREE-RADICALS; GLUTATHIONE; STRESS; PEROXIDATION; REDUCTASE; ASSAY;

D O I：

10.1002/jbt.21598

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The capability of lophirones B and C to extenuate aflatoxin B-1 (AFB(1))-mediated onslaught on cellular proteins, lipids, and DNA was investigated for 6 weeks. Lophirones B and C significantly (P < 0.05) increase the expression and specific activity of cytoprotective enzymes (glutathione-S-transferase, nioctinamide adenine dicludeotide: quinone oxidoreductase-1, epoxide hydrolase, and uridyl glucuronosyl transferase). There was significant (P < 0.05) reduction in the level of antioxidant system in AFB(1)-induced hepatocarcinogenesis. Furthermore, lophirones B and C significantly (P < 0.05) attenuated AFB(1)-mediated decrease in the specific activities of antioxidant enzymes. Oxidative stress biomarkers, malondialdehyde, lipid hydroperoxides, conjugated dienes, protein carbonyl, and fragmented DNA were significantly (P < 0.05) elevated in AFB(1)-treated rats. Although lophirones B and C did not significantly (P < 0.05) alter these biomarkers, an AFB(1)-mediated increase in these biomarkers was significantly attenuated. Results obtained showed that lophirones B and C extenuate AFB(1)-mediated onslaught on cellular proteins, lipids, and DNA by enhancing nuclear erythroid-related factor-2 expression. (C) 2014 Wiley Periodicals, Inc.

引用

页码：558 / 567

页数：10

共 52 条

[1] Cytotoxic, Antimutagenic, and Antioxidant Activities of Methanolic Extract and Chalcone Dimers (Lophirones B and C) Derived From Lophira alata (Van Tiegh. Ex Keay) Stem Bark [J].