RETRACTED: Lnc-SNHG1 Activates the TGFBR2/SMAD3 and RAB11A/Wnt/β-Catenin Pathway by Sponging MiR-302/372/373/520 in Invasive Pituitary Tumors (Publication with Expression of Concern. See vol. 56, pg. 87, 2022) (Retracted article)

被引：52
作者：
Wang, Heyuan [2 ,4 ]
Wang, Guixia [2 ]
Gao, Yufei [1 ]
Zhao, Conghai [1 ]
Li, Xiaoping [5 ]
Zhang, Fuqiang [6 ]
Jiang, Chunyan [7 ,8 ]
Wu, Bing [1 ,3 ]
机构：
[1] Jilin Univ, Dept Neurosurg, China Japan Union Hosp, Changchun, Jilin, Peoples R China
[2] Jilin Univ, Hosp 1, Dept Endocrinol & Metab, Changchun, Jilin, Peoples R China
[3] Jilin Univ, Key Lab Radiobiol, Minist Hlth Publ Hlth, Changchun, Jilin, Peoples R China
[4] Jilin Univ, Dept Immunol, Coll Basic Med Sci, Changchun, Jilin, Peoples R China
[5] Jilin Univ, Dept Pediat Endocrinol, Hosp 1, Changchun, Jilin, Peoples R China
[6] Jilin Univ, Sci & Res Ctr, China Japan Union Hosp, Tianjin, Peoples R China
[7] Tianjin Med Univ, Tianjin Metab Dis Hosp, Key Lab Hormones & Dev, Tianjin Key Lab Metab Dis,Minist Hlth, Tianjin, Peoples R China
[8] Tianjin Med Univ, Tianjin Inst Endocrinol, Tianjin, Peoples R China
来源：
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2018年 / 48卷 / 03期
关键词：
Lnc-SNHG1; TGFBR2; RAB11A; Wnt/beta-catenin; SMAD3; Pituitary tumor; LONG NONCODING RNAS; CELL-PROLIFERATION; SNHG1; PREDICTS; POOR-PROGNOSIS; LNCRNA SNHG1; CANCER; GROWTH; ADENOMAS; CONTRIBUTES; PROGRESSION;
D O I：
10.1159/000492089
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Background/Aims: Long noncoding RNAs (IncRNAs) are critical regulators in various diseases including human cancer and could function as competing endogenous RNAs (ceRNAs) to regulate microRNAs (miRNAs). Methods: Quantitative real-time PCR (qRT-PCR) was used to analyze the expression of Inc-SNHG1 and miR-302/372/373/520 in pituitary tumor tissues and cell lines. Cell proliferation was investigated using MTT and cell count assays. The mechanisms by which Inc-SNHG1 affects pituitary tumor progression were investigated using Western blot assays, transwell migration assays, immunohistochemistry, immunofluorescence, luciferase reporter assays, tumor xenografts, and flow cytometry. Results: We found that Inc-SNHG1 was overexpressed in invasive pituitary tumor tissues and cell lines. Ectopic expression of Inc-SNHG1 promoted cell proliferation, migration, and invasion, as well as the epithelial-mesenchymal transition (EMT), by affecting the cell cycle and cell apoptosis in vitro and tumor growth in vivo. Further study indicated that overexpression of Inc-SNHG1 markedly inhibited the expression of miR-302/372/373/520 (miRNA-pool) which is down-regulated in invasive pituitary tumor cells. Moreover, overexpression of Inc-SNHG1 significantly promoted the expression of TGFBR2 and RAB11A, the direct targets of miR-302/372/373/520. Finally, Inc-SNHG1 activates the TGFBR2/SMAD3 and RAB11A/Wnt/beta-catenin pathways in pituitary tumor cells via sponging miR-302/372/373/520. Conclusions: Our data suggest that Inc-SNHG1 promotes the progression of pituitary tumors and is a potential therapeutic target for invasive pituitary tumor. (C) 2018 The Author(s) Published by S Karger AG, Basel
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页码：1291 / 1303
页数：13
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