A large-scale systematic survey reveals recurring molecular features of public antibody responses to SARS-CoV-2

被引：61

作者：

Wang, Yiquan ^{[1
]}

Yuan, Meng ^{[2
]}

Lv, Huibin ^{[1
,3
]}

Peng, Jian ^{[4
]}

Wilson, Ian A. ^{[2
,5
]}

Wu, Nicholas C. ^{[1
,6
,7
,8
]}

机构：

[1] Univ Illinois, Dept Biochem, Urbana, IL 61801 USA

[2] Scripps Res Inst, Dept Integrat Struct & Computat Biol, La Jolla, CA 92037 USA

[3] Univ Hong Kong, Li Ka Shing Fac Med, Sch Publ Hlth, HKU Pasteur Res Pole, Hong Kong, Peoples R China

[4] Univ Illinois, Dept Comp Sci, Urbana, IL 61801 USA

[5] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

[6] Univ Illinois, Ctr Biophys & Quantitat Biol, Urbana, IL 61801 USA

[7] Univ Illinois, Carl R Woese Inst Genom Biol, Urbana, IL 61801 USA

[8] Univ Illinois, Carle Illinois Coll Med, Urbana, IL 61801 USA

来源：

IMMUNITY | 2022年 / 55卷 / 06期

基金：

美国国家卫生研究院;

关键词：

NEUTRALIZING ANTIBODIES; V(D)J RECOMBINATION; INFLUENZA-VIRUS; MECHANISMS; SPIKE; IDENTIFICATION; RECOGNITION; EPITOPES; DOMAIN;

D O I：

10.1016/j.immuni.2022.03.019

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Global research to combat the COVID-19 pandemic has led to the isolation and characterization of thousands of human antibodies to the SARS-CoV-2 spike protein, providing an unprecedented opportunity to study the antibody response to a single antigen. Using the information derived from 88 research publications and 13 patents, we assembled a dataset of ???8,000 human antibodies to the SARS-CoV-2 spike protein from >200 donors. By analyzing immunoglobulin V and D gene usages, complementarity-determining region H3 sequences, and somatic hypermutations, we demonstrated that the common (public) responses to different domains of the spike protein were quite different. We further used these sequences to train a deep-learning model to accurately distinguish between the human antibodies to SARS-CoV-2 spike protein and those to influenza hemagglutinin protein. Overall, this study provides an informative resource for antibody research and enhances our molecular understanding of public antibody responses.

引用

页码：1105 / +

页数：18

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