Differential MicroRNA Analyses of Burkholderia pseudomallei- and Francisella tularensis-Exposed hPBMCs Reveal Potential Biomarkers

被引：3

作者：

Cer, Regina Z. ^{[1
,2
]}

Herrera-Galeano, J. Enrique ^{[1
,2
,3
]}

Frey, Kenneth G. ^{[1
,2
]}

Schully, Kevin L. ^{[1
,2
]}

Luu, Truong V. ^{[1
,2
]}

Pesce, John ^{[1
,2
,4
]}

Mokashi, Vishwesh P. ^{[1
,5
]}

Keane-Myers, Andrea M. ^{[1
,2
,6
]}

Bishop-Lilly, Kimberly A. ^{[1
,2
]}

机构：

[1] Naval Med Res Ctr, Biol Def Res Directorate, Genom & Bioinformat Dept, Frederick, MD 21702 USA

[2] Henry M Jackson Fdn Adv Mil Med, Bethesda, MD 20814 USA

[3] KCE Serv & Consulting LLC, Columbia, MD USA

[4] NIAID, Div Microbiol & Infect Dis, 9000 Rockville Pike, Bethesda, MD 20892 USA

[5] Navy Drug Screening Lab, Jacksonville, FL USA

[6] NIH, Immunol, Bldg 10, Bethesda, MD 20892 USA

来源：

INTERNATIONAL JOURNAL OF GENOMICS | 2017年 / 2017卷

关键词：

GENE-EXPRESSION PROFILES; SYSTEM; IDENTIFICATION;

D O I：

10.1155/2017/6489383

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Increasing evidence that microRNAs (miRNAs) play important roles in the immune response against infectious agents suggests that miRNA might be exploitable as signatures of exposure to specific infectious agents. In order to identify potential early miRNA biomarkers of bacterial infections, human peripheral blood mononuclear cells (hPBMCs) were exposed to two select agents, Burkholderia pseudomallei K96243 and Francisella tularensis SHU S4, as well as to the nonpathogenic control Escherichia coli DH5a. RNA samples were harvested at three early time points, 30, 60, and 120 minutes postexposure, then sequenced. RNAseq analyses identified 87 miRNAs to be differentially expressed (DE) in a linear fashion. Of these, 31 miRNAs were tested using the miScript miRNA qPCR assay. Through RNAseq identification and qPCR validation, we identified differentially expressed miRNA species that may be involved in the early response to bacterial infections. Based upon its upregulation at early time points postexposure in two different individuals, hsa-mir-30c-5p is a miRNA species that could be studied further as a potential biomarker for exposure to these gram-negative intracellular pathogens. Gene ontology functional analyses demonstrated that programmed cell death is the first ranking biological process associated with miRNAs that are upregulated in F. tularensis-exposed hPBMCs.

引用

页数：13

共 35 条

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