Overgrowth (Della) mutants of wheat: development, growth and yield of intragenic suppressors of the Rht-B1c dwarfing gene

被引:8
作者
Derkx, Adinda P. [1 ]
Harding, Carol A. [1 ]
Miraghazadeh, Asemeh [1 ]
Chandler, Peter M. [1 ]
机构
[1] CSIRO Agr Flagship, GPO Box 1600, Canberra, ACT 2601, Australia
关键词
amino acid substitutions; mutation; plant height; Triticum aestivum; yield; SPRING WHEAT; MOLECULAR-MECHANISM; ISOGENIC LINES; USE EFFICIENCY; RECEPTOR GID1; PROTEIN SLR1; GIBBERELLIN; ARABIDOPSIS; LIGHT; RICE;
D O I
10.1071/FP16262
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A suppressor screen using the dwarf Rht-B1c Della mutant of wheat (Triticum aestivum L.) led to the isolation of overgrowth mutants, which retained the original dwarfing gene but grew at a faster rate because of a new mutation elsewhere in that gene. Forty-six alleles were identified, which included amino acid substitutions, premature stop codons, and splice site alterations. The sites of amino acid substitution were primarily localised around conserved motifs in the DELLA protein, and these mutants showed a wide range in their extent of growth recovery (dwarf, semidwarf, tall). Detailed growth comparisons were made on a wide height range of backcrossed overgrowth alleles, comparing stem and spike growth, leaf size, tillering, phenological development, coleoptile length, grain dormancy and grain yield. There were large and reproducible differences between alleles for some traits, whereas others were largely unaffected or varied with growth conditions. Some of the overgrowth alleles offer promise as alternatives to the Rht-B1b and Rht-D1b dwarfing genes, allowing a wider range of height control, improved grain dormancy and equivalent grain yield. The collection of mutants will also be valuable as a resource to study the effect of height on different physiological or agronomic traits, and in elucidating DELLA protein function.
引用
收藏
页码:525 / 537
页数:13
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