Light-Activated Decellularized Extracellular Matrix-Based Bioinks for Volumetric Tissue Analogs at the Centimeter Scale

被引:126
作者
Kim, Hyeonji [1 ]
Kang, Byeongmin [2 ]
Cui, Xiaolin [3 ,4 ]
Lee, Se-Hwan [2 ]
Lee, Kwangseok [1 ]
Cho, Dong-Woo [1 ]
Hwang, Woonbong [1 ]
Woodfield, Tim B. F. [3 ,4 ]
Lim, Khoon S. [3 ,4 ]
Jang, Jinah [1 ,2 ,5 ]
机构
[1] Pohang Univ Sci & Technol POSTECH, Dept Mech Engn, Pohang 37673, Kyungbuk, South Korea
[2] POSTECH, Dept Convergence IT Engn, Pohang 37673, Kyungbuk, South Korea
[3] Univ Otago, Dept Orthopaed Surg & Musculoskeletal Med, Christchurch 8011, New Zealand
[4] Med Technol Ctr Res Excellence MedTech CoRE, Auckland 1010, New Zealand
[5] POSTECH, Sch Interdisciplinary Biosci & Bioengn, Pohang 37673, Kyungbuk, South Korea
基金
新加坡国家研究基金会;
关键词
3D bioprinting technology; decellularized extracellular matrix; hydrogel; photopolymerization; scalable tissue manufacturing; tissue engineering; MECHANICAL-PROPERTIES; CELL-MIGRATION; CROSS-LINKING; COLLAGEN; RIBOFLAVIN; CONSTRUCTS; HYDROGELS;
D O I
10.1002/adfm.202011252
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Tissue engineering requires not only tissue-specific functionality but also a realistic scale. Decellularized extracellular matrix (dECM) is presently applied to the extrusion-based 3D printing technology. It has demonstrated excellent efficiency as bioscaffolds that allow engineering of living constructs with elaborate microarchitectures as well as the tissue-specific biochemical milieu of target tissues and organs. However, dECM bioinks have poor printability and physical properties, resulting in limited shape fidelity and scalability. In this study, new light-activated dECM bioinks with ruthenium/sodium persulfate (dERS) are introduced. The materials can be polymerized via a dityrosine-based cross-linking system with rapid reaction kinetics and improved mechanical properties. Complicated constructs with high aspect ratios can be fabricated similar to the geometry of the desired constructs with increased shape fidelity and excellent printing versatility using dERS. Furthermore, living tissue constructs can be safely fabricated with excellent tissue regenerative capacity identical to that of pure dECM. dERS may serve as a platform for a wider biofabrication window through building complex and centimeter-scale living constructs as well as supporting tissue-specific performances to encapsulated cells. This capability of dERS opens new avenues for upscaling the production of hydrogel-based constructs without additional materials and processes, applicable in tissue engineering and regenerative medicine.
引用
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页数:10
相关论文
共 59 条
[1]   Precise stacking of decellularized extracellular matrix based 3D cell-laden constructs by a 3D cell printing system equipped with heating modules [J].
Ahn, Geunseon ;
Min, Kyung-Hyun ;
Kim, Changhwan ;
Lee, Jeong-Seok ;
Kang, Donggu ;
Won, Joo-Yun ;
Cho, Dong-Woo ;
Kim, Jun-Young ;
Jin, Songwan ;
Yun, Won-Soo ;
Shim, Jin-Hyung .
SCIENTIFIC REPORTS, 2017, 7
[2]   Visible light mediated PVA-tyramine hydrogels for covalent incorporation and tailorable release of functional growth factors [J].
Atienza-Roca, Pau ;
Kieser, David C. ;
Cui, Xiaolin ;
Bathish, Boushra ;
Ramaswamy, Yogambha ;
Hooper, Gary J. ;
Clarkson, Andrew N. ;
Rnjak-Kovacina, Jelena ;
Martens, Penny J. ;
Wise, Lyn M. ;
Woodfield, Tim B. F. ;
Lim, Khoon S. .
BIOMATERIALS SCIENCE, 2020, 8 (18) :5005-5019
[3]  
Barker TH, 2001, J BIOMED MATER RES, V56, P529, DOI 10.1002/1097-4636(20010915)56:4<529::AID-JBM1124>3.0.CO
[4]  
2-2
[5]   Ruthenium-catalyzed photo cross-linking of fibrin-based engineered tissue [J].
Bjork, Jason W. ;
Johnson, Sandra L. ;
Tranquillo, Robert T. .
BIOMATERIALS, 2011, 32 (10) :2479-2488
[6]   Regulation of Keratocyte Phenotype and Cell Behavior by Substrate Stiffness [J].
Chen, Jialin ;
Backman, Ludvig J. ;
Zhang, Wei ;
Ling, Chen ;
Danielson, Patrik .
ACS BIOMATERIALS SCIENCE & ENGINEERING, 2020, 6 (09) :5162-5171
[7]   A 3D cell printed muscle construct with tissue-derived bioink for the treatment of volumetric muscle loss [J].
Choi, Yeong-Jin ;
Jun, Young-Joon ;
Kim, Dong Yeon ;
Yi, Hee-Gyeong ;
Chae, Su-Hun ;
Kang, Junsu ;
Lee, Juyong ;
Gao, Ge ;
Kong, Jeong-Sik ;
Jang, Jinah ;
Chung, Wan Kyun ;
Rhie, Jong-Won ;
Cho, Dong-Woo .
BIOMATERIALS, 2019, 206 :160-169
[8]   Advances in Extrusion 3D Bioprinting: A Focus on Multicomponent Hydrogel-Based Bioinks [J].
Cui, Xiaolin ;
Li, Jun ;
Hartanto, Yusak ;
Durham, Mitchell ;
Tang, Junnan ;
Zhang, Hu ;
Hooper, Gary ;
Lim, Khoon ;
Woodfield, Tim .
ADVANCED HEALTHCARE MATERIALS, 2020, 9 (15)
[9]   Rapid Photocrosslinking of Silk Hydrogels with High Cell Density and Enhanced Shape Fidelity [J].
Cui, Xiaolin ;
Soliman, Bram G. ;
Alcala-Orozco, Cesar R. ;
Li, Jun ;
Vis, Michelle A. M. ;
Santos, Miguel ;
Wise, Steven G. ;
Levato, Riccardo ;
Malda, Jos ;
Woodfield, Tim B. F. ;
Rnjak-Kovacina, Jelena ;
Lim, Khoon S. .
ADVANCED HEALTHCARE MATERIALS, 2020, 9 (04)
[10]   Decellularized extracellular matrix bioinks and the external stimuli to enhance cardiac tissue development in vitro [J].
Das, Sanskrita ;
Kim, Seok-Won ;
Choi, Yeong-Jin ;
Lee, Sooyeon ;
Lee, Se-Hwan ;
Kong, Jeong-Sik ;
Park, Hun-Jun ;
Cho, Dong-Woo ;
Jang, Jinah .
ACTA BIOMATERIALIA, 2019, 95 :188-200