Morphological, physiological and molecular responses of Nitzschia palea under cadmium stress

被引:16
作者
Tiam, Sandra Kim [1 ,2 ]
Lavoie, Isabelle [1 ]
Doose, Caroline [1 ]
Hamilton, Paul B. [3 ]
Fortin, Claude [1 ]
机构
[1] Inst Natl Rech Sci, 490 Rue Couronne, Quebec City, PQ G1K 9A9, Canada
[2] Museum Natl Hist Nat, CNRS Mol Commun & Adaptat Microorganismes Equipe, UMR MNHN 7245, 12 Rue Buffon, F-75231 Paris 05, France
[3] Canadian Museum Nat, Res & Collect, POB 3443Stn D, Ottawa, ON K1P 6P4, Canada
关键词
Cadmium; Teratologies; Diatoms; Bioaccumulation; Gene expression; qPCR; POLYCYCLIC AROMATIC-HYDROCARBONS; DIATOM THALASSIOSIRA-PSEUDONANA; METAL CONTAMINATION; MARINE DIATOM; PERIPHYTIC DIATOMS; GENE-EXPRESSION; COMMUNITIES; BIOFILM; POLLUTION; TOXICITY;
D O I
10.1007/s10646-018-1945-1
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
The impact of cadmium on the diatom Nitzschia palea (Kutzing) W. Smith 1856 was studied by examining the relation between valve deformities and response through biological processes and genetic expression. Cultures of N. palea were exposed to two Cd treatments (C-1 = 2.4 +/- 0.6 and C-2 = 42.6 +/- 4.2 A mu g Cd/L) along with a control (C-0 = 0 A mu g Cd/L) for 28 days. Cadmium bioaccumulation, diatoms growth, photosynthetic efficiencies, valve deformities and genetic expression were investigated during the course of the experiment. Cadmium exposure had significant effects on bioaccumulation, growth, valve deformities and genetic expression. Maximal effects for all studied endpoints were recorded after 7 days of exposure for the C-2 treatment, which corresponded to the sampling time and condition with maximum cadmium bioaccumulation. Abnormal raphe formations (deviation from its lateral position) were significantly more abundant in the C-2 treatment compared to the control. Molecular responses were related to cadmium level based on the number of genes impacted, intensity of the response and the frequency of observations. The expression of genes involved in the regulation of mitochondrial metabolism, photosynthesis, oxidative stress and silica metabolism was affected by cadmium exposure.
引用
收藏
页码:675 / 688
页数:14
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