Single cell screening approaches for antibody discovery

被引：34

作者：

Fitzgerald, Valerie ^{[1
]}

Leonard, Paul ^{[1
]}

机构：

[1] Vaccinogen Inc, 949 Fell St,2nd Floor, Baltimore, MD 21231 USA

来源：

METHODS | 2017年 / 116卷

关键词：

Antibody screening; Single cell analysis; Microtool; PHAGE DISPLAY TECHNOLOGY; MONOCLONAL-ANTIBODIES; MICROFLUIDIC DEVICE; RAPID SELECTION; MICROTOOLS; BACTERIA; CULTURE;

D O I：

10.1016/j.ymeth.2016.11.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Microtools that have been developed to allow in depth interrogation of individual cells in high throughput are improving our understanding of biological processes at the single cell level and are opening up new possibilities for biological research. In relation to antibody discovery, these tools are now helping to maximise the full potential of well-established methodologies for antibody generation. Being complementary to both recombinant and native antibody secreting cells, some of these tools are finding widespread use in the field. In this review, we discuss how microtools for single cell analysis are addressing some of the limitations of traditional approaches for antibody screening. We describe the main classes of microtools for antibody discovery along with a comparison of each technology and an outlook for the future utility of some of these microtools for discovery and research. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：34 / 42

页数：9

共 50 条

[41] Antibody Screening in Patients With Thalassemia Major [J].

Obeidi, Nrages ;

Mankhian, Ali Reza ;

Hatami, Gissoo ;

Emami, Habib .

LABMEDICINE, 2011, 42 (10) :618-621

[42] Application of microfluidic technology in antibody screening [J].

Sun, Heng ;

Hu, Ning ;

Wang, Jianhua .

BIOTECHNOLOGY JOURNAL, 2022, 17 (08)

[43] Modern approaches to chemical toxicity screening [J].

Hvastkovs, Eli G. ;

Rusling, James F. .

CURRENT OPINION IN ELECTROCHEMISTRY, 2017, 3 (01) :18-22

[44] Resonant waveguide grating imagers for single cell analysis and high throughput screening [J].

Fang, Ye .

BIOSENSING AND NANOMEDICINE VIII, 2015, 9550

[45] Microscale screening of antibody libraries as maytansinoid antibody-drug conjugates [J].

Catcott, Kalli C. ;

McShea, Molly A. ;

Bialucha, Carl Uli ;

Miller, Kathy L. ;

Hicks, Stuart W. ;

Saxena, Parmita ;

Gesner, Thomas G. ;

Woldegiorgis, Mikias ;

Lewis, Megan E. ;

Bai, Chen ;

Fleming, Michael S. ;

Ettenberg, Seth A. ;

Erickson, Hans K. ;

Yoder, Nicholas C. .

MABS, 2016, 8 (03) :513-523

[46] Isolation of functional single domain antibody by whole cell immunization: Implications for cancer treatment [J].

Baral, Toya Nath ;

Murad, Yanal ;

Thanh-Dung Nguyen ;

Iqbal, Umar ;

Zhang, Jianbing .

JOURNAL OF IMMUNOLOGICAL METHODS, 2011, 371 (1-2) :70-80

[47] Translating Cancer Molecular Variability into Personalized Information Using Bulk and Single Cell Approaches [J].

Kravchenko-Balasha, Nataly .

PROTEOMICS, 2020, 20 (13)

[48] Optical approaches for single-cell and subcellular analysis of GPCR–G protein signaling [J].

Dinesh Kankanamge ;

Kasun Ratnayake ;

Kanishka Senarath ;

Mithila Tennakoon ;

Elise Harmon ;

Ajith Karunarathne .

Analytical and Bioanalytical Chemistry, 2019, 411 :4481-4508

[49] Deciphering Myopia: Insights From Single-Cell Data and Machine Learning Approaches [J].

Hu, Junwei ;

Zhang, Yule ;

Piao, Yongjun .

2024 IEEE 9TH INTERNATIONAL CONFERENCE ON DATA SCIENCE IN CYBERSPACE, DSC, 2024, :782-788

[50] scNovel: a scalable deep learning-based network for novel rare cell discovery in single-cell transcriptomics [J].

Zheng, Chuanyang ;

Wang, Yixuan ;

Cheng, Yuqi ;

Wang, Xuesong ;

Wei, Hongxin ;

King, Irwin ;

Li, Yu .

BRIEFINGS IN BIOINFORMATICS, 2024, 25 (03)

← 1 2 3 4 5 →