Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses

被引:72
作者
Coad, Michael [1 ]
Clifford, Derek [1 ]
Rhodes, Shelley G. [1 ]
Hewinson, R. Glyn [1 ]
Vordermeier, H. Martin [1 ]
Whelan, Adam O. [1 ]
机构
[1] Vet Labs Agcy, TB Res Grp, Addlestone KT15 3NB, Surrey, England
关键词
bovine tuberculosis; skin-testing; gamma-interferon; interleukin-10; interleukin-1; beta; GAMMA-INTERFERON ASSAY; DELAYED-TYPE HYPERSENSITIVITY; INHIBITS CYTOKINE PRODUCTION; REGULATORY T-CELLS; MYCOBACTERIUM-BOVIS; DIAGNOSIS; IL-10; SENSITIVITY; PULMONARY;
D O I
10.1051/vetres/2009062
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The principal surveillance tool used to control bovine tuberculosis in cattle is the removal of animals that provide a positive response to the tuberculin skin-test. In this study we performed a longitudinal investigation of the immunological and diagnostic consequences of repeated short-interval skin-tests in cattle naturally infected with Mycobacterium bovis. Tuberculin skin-test positive cattle were subjected to up to four further intradermal comparative cervical skin-tests at approximately 60-day intervals. A significant progressive reduction in the strength of the skin-test was observed after successive tests. In contrast, the magnitude of interferon-gamma (IFN-gamma) responses was not influenced by repeat skin-testing either transiently around the time of each skin-test or longitudinally following repeated tests. A significant boost in blood interleukin-10 (IL-10) production was observed within 3 days following each skin-test although the magnitude of this boosted response returned to lower levels by day 10 post-test. The application of a novel multiplex assay to simultaneously measure seven cytokines and chemokines also identified that skin-testing resulted in a significant and progressive reduction in antigen specific interleukin-1 beta (IL-1 beta) whilst confirming stable IFN-gamma and elevated IL-10 responses in the blood. Therefore, we have demonstrated that in cattle naturally infected with M. bovis, repeat short-interval skin-testing can lead to a progressive reduction in skin-test responsiveness which has potential negative consequences for the detection of infected animals with marginal or inconclusive skin-test responses. The desensitising effect is associated with decreased IL-1 beta and elevated IL-10 responses, but importantly, does not influence antigen specific IFN-gamma responses.
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页数:12
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