Polyhydroxybutyrate production by Chlorella sorokiniana SVMIICT8 under Nutrient-deprived mixotrophy

Polyhydroxybutyrates (PHBs) are naturally occurring biopolymeric compounds that accumulate in a variety of microorganisms, including microalgae as energy and carbon storage sources. The present study was designed to evaluate nature-based PHB production using microalgae (Chlorella sorokiniana SVMIICT8) in biphasic (growth (GP) and stress phase (SP)) nutritional mode of cultivation. Microalgal PHB accumulation was driven by nutrient constraint, with a maximal production of 29.5% of PHB from 0.94 gm L-1 of biomass. Fluorescence microscopy revealed PHB granules in the cell cytoplasm, while NMR (1H and 13C), XRD and TGA analysis confirmed the structure. The biopolymer obtained was homopolymer of PHB with carbonyl (C=O) stretch of the aliphatic ester moiety. In GC-MS analysis, major peak representing butyric acid methyl ester also confirmed the PHB. Chlorophyll a fluorescence transients inferred through OJIP, exhibited significant variation in photosynthetic process during growth and nutrient limiting conditions. Mining of bio-based products from microalgae cultivation embrace nature-based approach addressing climate change and sustainability inclusively.