Sperm DNA fragmentation on the day of fertilization is not associated with embryologic or clinical outcomes after IVF/ICSI

被引：68

作者：

Green, Katherine A. ^{[1
]}

Patounakis, George ^{[2
]}

Dougherty, Michael P. ^{[3
]}

Werner, Marie D. ^{[4
]}

Scott, Richard T. ^{[4
]}

Franasiak, Jason M. ^{[4
]}

机构：

[1] Reprod Med Associates Southern Calif, 11500 West Olymp Blvd,Suite 150, Los Angeles, CA 90064 USA

[2] Reprod Med Associates Florida, 400 Colonial Ctr Pkwy,Suite 150, Lake Mary, FL 32746 USA

[3] Rutgers State Univ, Robert Wood Johnson Med Sch, Dept Obstet & Gynecol, 675 Hoes Lane West, Piscataway, NJ 08854 USA

[4] Reprod Med Associates New Jersey, 140 Allen Rd, Basking Ridge, NJ 07920 USA

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 2020年 / 37卷 / 01期

关键词：

Sperm; DNA fragmentation; IVF; Infertility; Male factor; CHROMATIN-STRUCTURE ASSAY; IN-VITRO FERTILIZATION; MEN; INFERTILITY; PREDICTION; PARAMETERS; INJECTION; DAMAGE; AGE;

D O I：

10.1007/s10815-019-01632-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose To evaluate if sperm DNA fragmentation (SDF) in the sample used for intracytoplasmic sperm injection (ICSI) impacts outcomes after euploid blastocyst transfer. Methods Prospective cohort study of couples undergoing IVF with preimplantation genetic testing for aneuploidy from December 2014-June 2017. Sperm collected on the day of ICSI was analyzed for SDF using the sperm chromatin structure assay (SCSA (R)). Semen analysis parameters, embryologic outcomes, and clinical outcomes after euploid blastocyst transfer were compared between groups with DNA fragmentation index (DFI) <= 15% and DFI > 15% using Mann-Whitney U, t tests, and generalized linear mixed effects models. Results Two hundred thirty-four patients were included. One hundred seventy-nine men had DFI <= 15% (low DFI group) and 55 men had DFI > 15% group (high DFI group). Total motile sperm and sperm concentration were significantly lower in the group with DFI > 15% vs. DFI <= 15%. There was no difference in fertilization (86.3 vs. 84.2%, adjusted OR (95% CI) 0.86 (0.63-1.18)), blastulation (49.5 vs. 48.8%, adjusted OR 1.02 (0.75-1.36)), or euploidy (55.7 vs. 52.1%, adjusted OR 0.96 (0.7-1.31)) between the low and high DFI groups, respectively. Clinical outcomes were similar between low and high DFI groups, including implantation rate (68.8 vs. 79.8%), ongoing pregnancy rate (65.9 vs. 72.6%), and miscarriage rate (4.2 vs. 8.8%), respectively. Conclusion Sperm DNA fragmentation on the day of ICSI is not associated with embryologic or clinical outcomes after euploid blastocyst transfer. Increasing levels of SDF are associated with low sperm concentration and total motile sperm count.

引用

页码：71 / 76

页数：6