Gal d 6 Is the Second Allergen Characterized from Egg Yolk

被引：49

作者：

Amo, Alvaro ^{[2
]}

Rodriguez-Perez, Rosa ^{[1
]}

Blanco, Juan ^{[2
]}

Villota, Julian ^{[1
]}

Juste, Sonsoles ^{[2
]}

Moneo, Ignacio ^{[1
]}

Luisa Caballero, Maria ^{[1
]}

机构：

[1] Hosp Carlos III, Dept Immunol, Madrid 28029, Spain

[2] Dept Allergol, Burgos 09006, Spain

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2010年 / 58卷 / 12期

关键词：

Egg allergy; food allergy; allergen detection; IgE; vitellogenin; CHICKEN SERUM-ALBUMIN; ANISAKIS-SIMPLEX; MAJOR ALLERGEN; BELUGA CAVIAR; IDENTIFICATION; VITELLOGENIN;

D O I：

10.1021/jf101403h

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Only one allergen from the egg yolk, alpha-livetin (Gal d 5) has been described thus far. A new egg yolk allergen was detected studying 27 egg allergic patients. The study was performed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and IgE-immunoblotting and IgE-immunoblotting-inhibition assays. An egg yolk extract was fractioned by reverse-phase high-performance liquid chromatography (RP-HPLC), and the new allergen detected was characterized by N-terminal amino acid analysis. A total of 5 of the 27 patients (18%) detected a yolk allergen of an apparent molecular weight of 35 kDa by SDS-PAGE. Heating and reduction treatments did not affect its allergenicity, although digestion with simulated gastric fluid diminished the IgE-binding capacity of the allergen. The N-terminal amino acid sequence corresponded with the YGP42 protein, a fragment of the vitellogenin-1 precursor. Thus, a second egg yolk allergen has been described and designated Gal d 6 by the World Health Organization (WHO)/International Union of Immunological Societies (IUIS) Allergen Nomenclature Subcommittee.

引用

页码：7453 / 7457

页数：5

共 13 条

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