Synergistic effect and efflux pump inhibitory activity of Ficus nitida phenolic extract with tetracycline against some pathogenic bacteria

被引:12
作者
Embaby, Mohamed A. [1 ,2 ]
El-Raey, Mohamed A. [3 ]
Zaineldain, Mohamed [4 ]
Almaghrabi, Omar [5 ]
Marrez, Diaa A. [2 ]
机构
[1] Univ Jeddah, Fac Sci & Arts Khulais, Dept Chem, Jeddah, Saudi Arabia
[2] Natl Res Ctr, Food Toxicol & Contaminants Dept, Cairo, Egypt
[3] Natl Res Ctr, Phytochem & Plant Systemat Dept, Cairo, Egypt
[4] October 6 Univ, Med Chem Dept, Fac Pharm, Giza, Egypt
[5] Univ Jeddah, Dept Biol, Fac Sci, Jeddah, Saudi Arabia
关键词
Synergistic effect; HPLC-PDA-MS; phenolic compounds; efflux pump inhibitors; molecular docking; ANTIMICROBIAL ACTIVITY; ANTIBACTERIAL ACTIVITY; TIME-KILL; ACID; MS/MS; MODE;
D O I
10.1080/15569543.2019.1659370
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Antibiotic resistance by pathogenic bacteria has risen to dangerous level all over the world. The study is aimed to evaluate the potential synergistic interaction of Ficus nitida bark phenolic extract with tetracycline against some pathogenic bacterial strains. The phenolic profile of the F. nitida bark acetone extract was determined using HPLC-PDA-MS/MS and the synergistic interaction of the acetone extract with tetracycline was evaluated by checkerboard assay followed by molecular docking. Fourteen phenolic compounds were identified in the acetone extract that include quinic acid and its derivatives in addition to flavan-3-ol and procyanidin derivatives. Synergistic interaction between the acetone extract and tetracycline was investigated against Bacillus cereus, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa with fraction inhibitory concentration index (FICI) values of 0.43, 0.50, 0.38, and 0.375, respectively and the additive effect against Salmonella typhi and Klebsiella pneumonia with FICI value of 0.625 and 0.75, respectively. In silico molecular docking study of some acetone fractions, namely; 5-caffeoyl quinic acid, Catechin-O- trihydroxy phenyl rhamnoside, Cinchonain, and Equisetumpyrone indicated that these constituents exhibited synergistic effect due to their efflux pump inhibitory effect. Significant enhancement in antibacterial activity was observed against both Gram positive and Gram negative bacteria due to combination of F. nitida phenolic extract with tetracycline. Thus, can be used as new antibiotic agent in pharmaceutical and veterinary industries.
引用
收藏
页码:1187 / 1197
页数:11
相关论文
共 52 条
[1]  
Acosta C.J.H., 2013, CURR RES BIO PHARM S, V2, P22
[2]  
Adebayo-Tayo B. C., 2012, African Journal of Biomedical Research, V15, P35
[3]   In vitro antimicrobial and resistance-modifying activities of aqueous crude khat extracts against oral microorganisms [J].
Al-hebshi, N ;
Al-haroni, M ;
Skaug, N .
ARCHIVES OF ORAL BIOLOGY, 2006, 51 (03) :183-188
[4]   Determination of minimum inhibitory concentrations [J].
Andrews, JM .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2001, 48 :5-16
[5]  
[Anonymous], 1999, The SAS/STAT User's guide-release, V8th, P1028
[6]   Role of hydrogen peroxide in bactericidal action of catechin [J].
Arakawa, H ;
Maeda, M ;
Okubo, S ;
Shimamura, T .
BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2004, 27 (03) :277-281
[7]   5-O-Caffeoylquinic acid: A spectroscopic study and biological screening for antimicrobial activity [J].
Bajko, Ewelina ;
Kalinowska, Monika ;
Borowski, Piotr ;
Siergiejczyk, Leszek ;
Lewandowski, Wlodzimierz .
LWT-FOOD SCIENCE AND TECHNOLOGY, 2016, 65 :471-479
[8]  
BAUER AW, 1966, AM J CLIN PATHOL, V45, P493
[9]   Tetracycline antibiotics: Mode of action, applications, molecular biology, and epidemiology of bacterial resistance [J].
Chopra, I ;
Roberts, M .
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2001, 65 (02) :232-+
[10]   INVESTIGATIONS INTO THE MECHANISM OF ACTION OF THE ANTIBACTERIAL AGENT NORFLOXACIN [J].
CRUMPLIN, GC ;
KENWRIGHT, M ;
HIRST, T .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1984, 13 :9-23