Identification of two novel Trichomonas vaginalis eif-5a genes

被引：12

作者：

Carvajal-Gamez, B. ^{[1
]}

Arroyo, R. ^{[2
]}

Lira, R. ^{[3
]}

Lopez-Camarillo, C. ^{[1
]}

Alvarez-Sanchez, M. E. ^{[1
]}

机构：

[1] UACM, Mexico City 03100, DF, Mexico

[2] CINVESTAV IPN, Dept Infectom & Patogenesis Mol, Mexico City 07360, Col San Pedro Z, Mexico

[3] IMSS, Unidad Enfermedades Infecciosas & Parasitarias, Mexico City 06720, Col Doctores, Mexico

来源：

INFECTION GENETICS AND EVOLUTION | 2010年 / 10卷 / 02期

关键词：

Eukaryotic translation initiation factor 5A (eIF-5A); Polyamines; Hypusine; Trichomonas vaginalis; TRANSLATION INITIATION-FACTOR; ELONGATION-FACTOR-P; FACTOR; 5A; TRICHOMONAS-VAGINALIS; DEOXYHYPUSINE SYNTHASE; POSTTRANSLATIONAL SYNTHESIS; SACCHAROMYCES-CEREVISIAE; HYPUSINE MODIFICATION; PROTEIN-SYNTHESIS; FACTOR EIF-5A;

D O I：

10.1016/j.meegid.2009.12.008

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The eukaryotic translation initiation factor 5A (eIF-5A) is highly conserved and is the only protein that is known to contain the unique and essential amino acid residue hypusine. Synthesis of hypusine is essential for the function of eIF5A in eukaryotic cell proliferation and survival. In this study, we identified two novel eukaryotic translation initiation factor 5A (eIF-5A) genes in Trichomonas vaginalis. The tveif-5a1 and tveif-5a2 putative genes were localized in different contigs, both containing ORFs encoding proteins of 168 amino acids that share high sequence identity with eIF-5A sequences from other eukaryotic organisms. A phylogenetic tree constructed with TveIF-5A1 and TveIF-5A2 from T. vaginalis and 13 other eIF-5A sequences of eukaryotic and archaebacterial origin revealed that both trichomonal TveIF-5As show the highest degree of similarity to bacteria. Using an anti-TveIF-5A antibody, we detected two protein bands and spots of 19 and 20 kDa with isoelectric points (pI) of 5.2 and 5.5, respectively, by one and two-dimensional Western blot assays. In addition, we used reverse transcription polymerase chain reaction (RTPCR) to demonstrate that both of these tveif-5a genes are expressed in T. vaginalis. Immunofluorescence assays showed that the TveIF-5A protein was dispersed throughout the parasite cytoplasm. In conclusion, T. vaginalis has two eif-5a genes, and both genes are expressed as highly conserved proteins of 19 kDa, which are localized in the cytoplasm of this parasite. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：284 / 291

页数：8

共 49 条

[1] Negative iron regulation of the CP65 cysteine proteinase cytotoxicity in Trichomonas vaginalis [J].