IgA Antibody Response of Swine to Foot-and-Mouth Disease Virus Infection and Vaccination

被引:29
作者
Pacheco, Juan M. [1 ]
Butler, John E. [2 ]
Jew, Jessica [2 ]
Ferman, Geoffrey S. [1 ]
Zhu, James [1 ]
Golde, William T. [1 ]
机构
[1] ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY 11944 USA
[2] Univ Iowa, Coll Med, Dept Microbiol, Iowa City, IA 52242 USA
关键词
NEONATAL PIGLETS; IMMUNE-RESPONSES; REPERTOIRE DEVELOPMENT; MONOCLONAL-ANTIBODIES; CATTLE; LOCALIZATION; TRANSMISSION; RESISTANCE; PATTERNS; CARRIER;
D O I
10.1128/CVI.00429-09
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Foot-and-mouth disease virus (FMDV) continues to be a significant economic problem worldwide. Control of the disease involves the use of killed-virus vaccines, a control measure developed decades ago. After natural infection, the primary site of replication of FMDV is the pharyngeal area, suggesting that a mucosal immune response is the most effective. Humoral immunity to killed-virus vaccination induces antibodies that can prevent the clinical disease but not local infection. Determining whether infection or vaccination stimulates IgA-mediated local immunity depends on the method of analysis. Different assays have been described to analyze the quality of antibody responses of cattle and swine to FMDV, including indirect double-antibody sandwich enzyme-linked immunosorbent assay (IDAS-ELISA) and antibody capture assay-ELISA (ACA-ELISA). We tested these assays on swine and show that vaccinated animals had FMDV-specific IgM and IgG but no IgA in either serum or saliva. After the infection, both assays detected FMDV-specific IgM, IgG, and IgA in serum. Notably, serum IgA was more readily detected using the ACA-ELISA, whereas IgA was not detected in saliva with this assay. FMDV-specific IgA antibodies were detected in saliva samples using the IDAS-ELISA. These data show that parenterally administered, killed-virus vaccine does not induce a mucosal antibody response to FMDV and illuminates limitations and appropriate applications of the two ELISAs used to measure FMDV-specific responses. Further, the presence of the IgA antivirus in serum correlates with the presence of such antibodies in saliva.
引用
收藏
页码:550 / 558
页数:9
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