Addition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock: effects on the production of reactive oxygen species, mitochondrial activity and oocyte competence

被引:37
作者
Ascari, I. J. [1 ]
Alves, N. G. [1 ]
Jasmin, J. [2 ]
Lima, R. R. [3 ]
Quintao, C. C. R. [4 ]
Oberlender, G. [5 ]
Moraes, E. A. [6 ]
Camargo, L. S. A. [4 ]
机构
[1] Univ Fed Lavras, Dept Anim Sci, Lavras, MG, Brazil
[2] Univ Fed Rio de Janeiro, NUMPEX Bio, Campus Duque de Caxias, Rio De Janeiro, Brazil
[3] Univ Fed Lavras, Dept Exact Sci, Lavras, Brazil
[4] Brazilian Agr Res Corp Dairy Cattle, Juiz De Fora, MG, Brazil
[5] Fed Inst Educ Sci & Technol South Minas Gerais, Muzambinho, MG, Brazil
[6] Fed Univ Vale Sao Francisco, Sch Anim Sci, Petrolina, PE, Brazil
关键词
Thermoprotection; Heat stress; DNA fragmentation; Mitochondrial membrane potential; ROS production; DEVELOPMENTAL COMPETENCE; BOS-INDICUS; VITRO MATURATION; OXIDATIVE STRESS; PREIMPLANTATION EMBRYOS; MOLECULAR-BASIS; APOPTOSIS; TAURUS; CATTLE; BLASTOCYSTS;
D O I
10.1016/j.domaniend.2017.03.003
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 hat 38.5 degrees C, or heat shock: 12 h at 41 degrees C followed by 12 h at 38.5 degrees C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM medium with IGF-I may increase the mitochondrial membrane potential and ROS production in oocytes and decrease apoptosis in the ICM in blastocysts. Heat shock for 12 h compromised oocyte developmental competence and increased apoptosis within the ICM cells of the blastocysts. (C) 2017 Elsevier Inc. All rights reserved.
引用
收藏
页码:50 / 60
页数:11
相关论文
共 65 条
[21]   Insulin-like growth factor-I promotes resistance of bovine preimplantation embryos to heat shock through actions independent of its anti-apoptotic actions requiring PI3K signaling [J].
Jousan, F. Dean ;
Hansen, Peter J. .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 2007, 74 (02) :189-196
[22]   Insulin-like growth factor-I as a survival factor for the bovine preimplantation embryo exposed to heat shock [J].
Jousan, FD ;
Hansen, PJ .
BIOLOGY OF REPRODUCTION, 2004, 71 (05) :1665-1670
[23]   Heat shock reduces developmental competence and alters spindle configuration of bovine oocytes [J].
Ju, JC ;
Jiang, S ;
Tseng, JK ;
Parks, JE ;
Yang, XZ .
THERIOGENOLOGY, 2005, 64 (08) :1677-1689
[24]   Effects of Serum-Free In Vitro Maturation of Bovine Oocytes on Subsequent Embryo Development and Cell Allocation in Two Developmental Stages of Day 7 Blastocysts [J].
Korhonen, K. ;
Kananen, K. ;
Ketoja, E. ;
Matomaki, J. ;
Halmekyto, M. ;
Peippo, J. .
REPRODUCTION IN DOMESTIC ANIMALS, 2010, 45 (01) :42-49
[25]   Growth factors protect in vitro cultured embryos from the consequences of oxidative stress [J].
Kurzawa, R ;
Glabowski, W ;
Baczkowski, T ;
Wiszniewska, B ;
Marchlewicz, M .
ZYGOTE, 2004, 12 (03) :231-240
[26]  
Lima RS, 2013, BRAZ J VET RES ANIM, V50, P264
[27]  
Lima RS, 2016, REPROD FERTIL DEV
[28]   Effect of holding equine oocytes in meiosis inhibitor-free medium before in vitro maturation and of holding temperature on meiotic suppression and mitochondrial energy/redox potential [J].
Martino, Nicola A. ;
Dell'Aquila, Maria E. ;
Uranio, Manuel Filioli ;
Rutigliano, Lucia ;
Nicassio, Michele ;
Lacalandra, Giovanni M. ;
Hinrichs, Katrin .
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 2014, 12
[29]   Vitrification preserves chromatin integrity, bioenergy potential and oxidative parameters in mouse embryos [J].
Martino, Nicola A. ;
Dell'Aquila, Maria E. ;
Cardone, Rosa A. ;
Somoskoi, Bence ;
Lacalandra, Giovanni M. ;
Cseh, Sandor .
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 2013, 11
[30]   Oocyte mitochondrial bioenergy potential and oxidative stress: within-/between-subject, in vivo versus in vitro maturation, and age-related variations in a sheep model [J].
Martino, Nicola Antonio ;
Lacalandra, Giovanni Michele ;
Uranio, Manuel Filioli ;
Ambruosi, Barbara ;
Caira, Michele ;
Silvestre, Fabio ;
Pizzi, Flavia ;
Desantis, Salvatore ;
Accogli, Gianluca ;
Dell'Aquila, Maria Elena .
FERTILITY AND STERILITY, 2012, 97 (03) :720-U230