A functional genomics screen identifying blood cell development genes in Drosophila by undergraduates participating in a course-based research experience

被引：8

作者：

Evans, Cory J. ^{[1
,2
,7
]}

Olson, John M. ^{[1
,8
]}

Mondal, Bama Charan ^{[1
,9
]}

Kandimalla, Pratyush ^{[1
,2
]}

Abbasi, Ariano ^{[1
]}

Abdusamad, Mai M. ^{[1
]}

Acosta, Osvaldo ^{[1
]}

Ainsworth, Julia A. ^{[1
]}

Akram, Haris M. ^{[1
]}

Albert, Ralph B. ^{[1
]}

Alegria-Leal, Elitzander ^{[1
]}

Alexander, Kai Y. ^{[1
]}

Ayala, Angelica C. ^{[1
]}

Balashova, Nataliya S. ^{[1
]}

Barber, Rebecca M. ^{[1
]}

Bassi, Harmanjit ^{[1
]}

Bennion, Sean P. ^{[1
]}

Beyder, Miriam ^{[1
]}

Bhatt, Kush, V ^{[1
]}

Bhoot, Chinmay ^{[1
]}

Bradshaw, Aaron W. ^{[1
]}

Brannigan, Tierney G. ^{[1
]}

Cao, Boyu ^{[1
]}

Cashell, Yancey Y. ^{[1
]}

Chai, Timothy ^{[1
]}

Chan, Alex W. ^{[1
]}

Chan, Carissa ^{[1
]}

Chang, Inho ^{[1
]}

Chang, Jonathan ^{[1
]}

Chang, Michael T. ^{[1
]}

Chang, Patrick W. ^{[1
]}

Chang, Stephen ^{[1
]}

Chari, Neel ^{[1
]}

Chassiakos, Alexander J. ^{[1
]}

Chen, Iris E. ^{[1
]}

Chen, Vivian K. ^{[1
]}

Chen, Zheying ^{[1
]}

Cheng, Marsha R. ^{[1
]}

Chiang, Mimi ^{[1
]}

Chiu, Vivian ^{[1
]}

Choi, Sharon ^{[1
]}

Chung, Jun Ho ^{[1
]}

Contreras, Liset ^{[1
]}

Corona, Edgar ^{[1
]}

Cruz, Courtney J. ^{[1
]}

Cruz, Renae L. ^{[1
]}

Dang, Jefferson M. ^{[1
]}

Dasari, Suhas P. ^{[1
]}

De la Fuente, Justin R. O. ^{[1
]}

Del Rio, Oscar M. A. ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Biomed Res Minor, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Ctr Adv Teaching, Los Angeles, CA 90095 USA

[4] Grinnell Coll, Dept Psychol, Grinnell, IA 50112 USA

[5] Univ Calif Los Angeles, Dept Biol Chem, Los Angeles, CA 90095 USA

[6] Univ Calif Los Angeles, UCLA Broad Stem Cell Res Ctr, Los Angeles, CA 90095 USA

[7] Loyola Marymount Univ, Dept Biol, Los Angeles, CA 90045 USA

[8] Cold Spring Harbor Asia, DNA Learning Ctr, Suzhou, Jiangsu, Peoples R China

[9] Banaras Hindu Univ, Inst Sci, Dept Zool, Cytogenet Lab, Varanasi 221005, Uttar Pradesh, India

来源：

G3-GENES GENOMES GENETICS | 2021年 / 11卷 / 01期

关键词：

hematopoiesis; blood; RNAi; education; CURE; HEMATOPOIETIC PROGENITORS; LINEAGE SPECIFICATION; RNA INTERFERENCE; LYMPH-GLAND; DUAL ROLE; NOTCH; DIFFERENTIATION; EXPRESSION; NICHE; MELANOGASTER;

D O I：

10.1093/g3journal/jkaa028

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Undergraduate students participating in the UCLA Undergraduate Research Consortium for Functional Genomics (URCFG) have conducted a two-phased screen using RNA interference (RNAi) in combination with fluorescent reporter proteins to identify genes important for hematopoiesis in Drosophila. This screen disrupted the function of approximately 3500 genes and identified 137 candidate genes for which loss of function leads to observable changes in the hematopoietic development. Targeting RNAi to maturing, progenitor, and regulatory cell types identified key subsets that either limit or promote blood cell maturation. Bioinformatic analysis reveals gene enrichment in several previously uncharacterized areas, including RNA processing and export and vesicular trafficking. Lastly, the participation of students in this course-based undergraduate research experience (CURE) correlated with increased learning gains across several areas, as well as increased STEM retention, indicating that authentic, student-driven research in the form of a CURE represents an impactful and enriching pedagogical approach.

引用

页数：23

共 94 条

[41] Mutations in the heatshock cognate 70 protein (hsc4) modulate Notch signaling
Hing, HK
Bangalore, L
Sun, X
Artavanis-Tsakonas, S
[J]. EUROPEAN JOURNAL OF CELL BIOLOGY, 1999, 78 (10) : 690 - 697
[42] Diversifying Science: Underrepresented Student Experiences in Structured Research Programs
Hurtado, Sylvia
Cabrera, Nolan L.
Lin, Monica H.
Arellano, Lucy
Espinosa, Lorelle L.
[J]. RESEARCH IN HIGHER EDUCATION, 2009, 50 (02) : 189 - 214
[43] A Broadly Implementable Research Course in Phage Discovery and Genomics for First-Year Undergraduate Students
Jordan, Tuajuanda C.
Burnett, Sandra H.
Carson, Susan
Caruso, Steven M.
Clase, Kari
DeJong, Randall J.
Dennehy, John J.
Denver, Dee R.
Dunbar, David
Elgin, Sarah C. R.
Findley, Ann M.
Gissendanner, Chris R.
Golebiewska, Urszula P.
Guild, Nancy
Hartzog, Grant A.
Grillo, Wendy H.
Hollowell, Gail P.
Hughes, Lee E.
Johnson, Allison
King, Rodney A.
Lewis, Lynn O.
Li, Wei
Rosenzweig, Frank
Rubin, Michael R.
Saha, Margaret S.
Sandoz, James
Shaffer, Christopher D.
Taylor, Barbara
Temple, Louise
Vazquez, Edwin
Ware, Vassie C.
Barker, Lucia P.
Bradley, Kevin W.
Jacobs-Sera, Deborah
Pope, Welkin H.
Russell, Daniel A.
Cresawn, Steven G.
Lopatto, David
Bailey, Cheryl P.
Hatfull, Graham F.
[J]. MBIO, 2014, 5 (01):
[44] The Drosophila lymph gland as a developmental model of hematopoiesis
Jung, SH
Evans, CJ
Uemura, C
Banerjee, U
[J]. DEVELOPMENT, 2005, 132 (11): : 2521 - 2533
[45] Lar maintains the homeostasis of the hematopoietic organ in Drosophila by regulating insulin signaling in the niche
Kaur, Harleen
Sharma, Shiv Kumar
Mandal, Sudip
Mandal, Lolitika
[J]. DEVELOPMENT, 2019, 146 (24):
[46] Control of blood cell homeostasis in Drosophila larvae by the posterior signalling centre
Krzemien, Joanna
Dubois, Laurence
Makki, Rami
Meister, Marie
Vincent, Alain
Crozatier, Michele
[J]. NATURE, 2007, 446 (7133) : 325 - 328
[47] Nuclear Pore Proteins in Regulation of Chromatin State
Kuhn, Terra M.
Capelson, Maya
[J]. CELLS, 2019, 8 (11)
[48] A serrate-expressing signaling center controls Drosophila hematopoiesis
Lebestky, T
Jung, SH
Banerjee, U
[J]. GENES & DEVELOPMENT, 2003, 17 (03) : 348 - 353
[49] Specification of Drosophila hematopoietic lineage by conserved transcription factors
Lebestky, T
Chang, T
Hartenstein, V
Banerjee, U
[J]. SCIENCE, 2000, 288 (5463) : 146 - 149
[50] Drosophila sessile hemocyte clusters are true hematopoietic tissues that regulate larval blood cell differentiation
Leitao, Alexandre B.
Sucena, Elio
[J]. ELIFE, 2015, 4 : 1 - 38

← 1 2 3 4 5 6 7 8 9 10 →