CRISPR-Cas9 and beyond: what's next in plant genome engineering

被引:14
作者
Zess, Erin [1 ]
Begemann, Matthew [2 ]
机构
[1] Carnegie Inst Sci, Dept Plant Biol, 290 Panama St, Stanford, CA 94305 USA
[2] Benson Hill Inc, 1100 Corp Sq Dr, St Louis, MO 63121 USA
关键词
Genome Editing; CRISPR Cas; IN-VIVO; STREPTOCOCCUS-THERMOPHILUS; HOMOLOGOUS RECOMBINATION; TARGETED MUTAGENESIS; ANALYSIS REVEALS; TAL EFFECTORS; DNA; RNA; NUCLEASES; CAS9;
D O I
10.1007/s11627-021-10185-1
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Scientists have developed and deployed successive generations of genome engineering technologies for use in plants, including meganucleases, zinc finger nucleases, TAL effector nucleases, and CRISPR nucleases. Each of these tools has been hailed as potentially revolutionary, capable of providing more efficient and precise ways to modify plant genomes toward improving agronomic traits or making fundamental discoveries. The CRISPR nucleases, in particular, have accelerated the pace of innovation and expanded the boundaries of what is achievable within the plant research space. This review will take care to discuss current plant genome engineering technologies, covering both well-established and up-and-coming tools, as well as describe potential and real-world applications.
引用
收藏
页码:584 / 594
页数:11
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