Hemostasis and Anti-Inflammatory Abilities of AuNPs-Coated Chitosan Dressing for Burn Wounds

被引:21
作者
Cherng, Juin-Hong [1 ,2 ]
Lin, Cheng-An J. [3 ,4 ]
Liu, Cheng-Che [5 ]
Yeh, Jue-Zong [6 ]
Fan, Gang-Yi [2 ,7 ]
Tsai, Hsin-Da [7 ]
Chung, Chun-Fang [7 ]
Hsu, Sheng-Der [8 ]
机构
[1] Natl Def Med Ctr, Grad Inst Life Sci, Taipei 114, Taiwan
[2] Natl Def Med Ctr, Dept & Grad Inst Biol & Anat, Taipei 114, Taiwan
[3] Chung Yuan Christian Univ, Dept Biomed Engn, Taoyuan 320, Taiwan
[4] Chung Yuan Christian Univ, Ctr Biomed Engn Canc, Taoyuan 320, Taiwan
[5] Natl Def Med Ctr, Grad Inst Physiol, Dept Physiol & Biophys, Taipei 114, Taiwan
[6] Triserv Gen Hosp, Natl Def Med Ctr, Dept Pharm, Taipei 114, Taiwan
[7] Natl Def Med Ctr, Lab Adult Stem Cell & Tissue Regenerat, Taipei 114, Taiwan
[8] Triserv Gen Hosp, Natl Def Med Ctr, Dept Surg, Div Traumatol, Taipei 114, Taiwan
关键词
chitosan; AuNPs; burn; wound dressing; healing; inflammation; hemostasis; GROWTH-FACTOR-C; GOLD NANOPARTICLES; KAPPA-B; KEAP1-NRF2; PATHWAY; SIGNALING PATHWAY; GENE-TRANSFER; ANGIOGENESIS; MECHANISMS; HYDROGEL; THROMBOMODULIN;
D O I
10.3390/jpm12071089
中图分类号
R19 [保健组织与事业(卫生事业管理)];
学科分类号
摘要
Burn injuries are a common hazard in the military, as fire is likely to be weaponized. Thus, it is important to find an effective substance to accelerate burn wound healing. This study used chitosan and gold nanoparticles (AuNPs) as wound dressings and investigated their effectiveness in femoral artery hemorrhage swine and rat burn models. Chitosan dressing has significant hemostatic properties compared with gauze. Histological results showed that burn wounds treated with chitosan or AuNP-coated chitosan dressings exhibited more cells and a continuous structure of the epidermis and dermis than those of the control and untreated lesion groups. Furthermore, both chitosan dressings have been shown to positively regulate the expression of genes- and cytokines/chemokines-related to the wound healing process; AuNP-coated chitosan significantly lessened severe sepsis and inflammation, balanced the activities of pro-fibrotic and anti-fibrotic ligands for tissue homeostasis, regulated angiogenesis, and inhibited apoptosis activity, thereby being beneficial for the burn microenvironment. Hence, chitosan alone or in combination with AuNPs represents a prospective therapeutic substance as a burn dressing which might be helpful for burn wound care. This study provides a novel hemostasis dressing for modern warfare that is simple to use by most medical and paramedical personnel handling for burn treatment.
引用
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页数:17
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