Crystal structure of lipocalin-type prostaglandin D synthase

Lipocalin-type prostaglandin (PG) D synthase (L-PGDS) is responsible for the biosynthesis of PGD(2), a potent endogenous sleep-inducing substance found in the central nervous system. In this study, we crystallized recombinant mouse L-PGDS from a sodium citrate solution by the hanging drop vapor-diffusion method. The crystal structure of the selenomethionyl Cys(65) Ala-substituted L-PGDS was determined at 2.5 Angstrom resolution by using the multiwavelength anomalous diffraction method. The crystals belonged to the orthorhombic space group C222(1) with lattice constants a = 45.5, b = 66.8, c = 104.5 Angstrom. The overall architecture of L-PGDS had an eight-stranded beta-barrel with a hydrophobic cavity, in which the Cys(65) residue, an active site for the catalysis, was identified. (C) 2002 Elsevier Science B.V. All rights reserved.