A LysM Receptor Heteromer Mediates Perception of Arbuscular Mycorrhizal Symbiotic Signal in Rice

被引：115

作者：

He, Jiangman ^{[1
,2
]}

Zhang, Chi ^{[1
,2
]}

Dai, Huiling ^{[1
]}

Liu, Huan ^{[1
,2
]}

Zhang, Xiaowei ^{[1
]}

Yang, Jun ^{[1
]}

Chen, Xi ^{[1
,2
]}

Zhu, Yayun ^{[3
]}

Wang, Dapeng ^{[1
,4
]}

Qi, Xiaofeng ^{[1
,2
]}

Li, Weichao ^{[1
,2
]}

Wang, Zhihui ^{[1
,2
]}

An, Guoyong ^{[4
]}

Yu, Nan ^{[3
]}

He, Zuhua ^{[1
]}

Wang, Yong-Fei ^{[1
]}

Xiao, Youli ^{[1
]}

Zhang, Peng ^{[1
]}

Wang, Ertao ^{[1
]}

机构：

[1] Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Natl Key Lab Plant Mol Genet, Inst Plant Physiol & Ecol,Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100039, Peoples R China

[3] Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 200234, Peoples R China

[4] Henan Univ, Collaborat Innovat Ctr Crop Stress Biol, State Key Lab Cotton Biol, Inst Plant Stress Biol,Dept Biol, Kaifeng 475001, Henan, Peoples R China

来源：

MOLECULAR PLANT | 2019年 / 12卷 / 12期

关键词：

rice; arbuscular mycorrhizal symbiosis; Myc factors; Nod factors; LysM receptor-like kinases; MEDICAGO-TRUNCATULA; NODULATION FACTORS; GENE-EXPRESSION; TRANSCRIPTION FACTOR; CALCIUM SPIKING; KINASE GENE; RECOGNITION; OSCERK1; ARABIDOPSIS; MOLECULES;

D O I：

10.1016/j.molp.2019.10.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Symbiotic microorganisms improve nutrient uptake by plants. To initiate mutualistic symbiosis with arbuscular mycorrhizal (AM) fungi, plants perceive Myc factors, including lipochitooligosaccharides (LCOs) and short-chain chitooligosaccharides (CO4/CO5), secreted by AM fungi. However, the molecular mechanism of Myc factor perception remains elusive. In this study, we identified a heteromer of LysM receptor-like kinases consisting of OsMYR1/OsLYK2 and OsCERK1 that mediates the perception of AM fungi in rice. CO4 directly binds to OsMYR1, promoting the dimerization and phosphorylation of this receptor complex. Compared with control plants, Osmyr1 and Oscerk1 mutant rice plants are less sensitive to Myc factors and show decreased AM colonization. We engineered transgenic rice by expressing chimeric receptors that respectively replaced the ectodomains of OsMYR1 and OsCERK1 with those from the homologous Nod factor receptors MtNFP and MtLYK3 of Medicago truncatula. Transgenic plants displayed increased calcium oscillations in response to Nod factors compared with control rice. Our study provides significant mechanistic insights into AM symbiotic signal perception in rice. Expression of chimeric Nod/Myc receptors achieves a potentially important step toward generating cereals that host nitrogen-fixing bacteria.

引用

页码：1561 / 1576

页数：16

共 92 条

[1] Quantitative RT-PCR analysis of 20 transcription factor genes of MADS, ARF, HAP2, MBF and HB families in moisture stressed shoot and root tissues of sorghum [J].