Attenuation of high cholesterol-induced oxidative stress in rabbit liver by thymoquinone

被引:25
作者
Attia, Ahmed [2 ]
Ragheb, Ahmed [2 ]
Sylwestrowicz, Thomas
Shoker, Ahmed [1 ,2 ]
机构
[1] Univ Saskatchewan, Dept Med, Div Nephrol, Transplant Program, Saskatoon, SK S7N 0W8, Canada
[2] Univ Saskatchewan, Dept Med, Royal Univ Hosp, Saskatoon, SK S7N 0W8, Canada
关键词
hyperlipidemia; oxidative stress; rabbit; steatosis; thymoquinone; NONALCOHOLIC STEATOHEPATITIS; LIPID-PEROXIDATION; CARBON-TETRACHLORIDE; NUTRITIONAL MODEL; VITAMIN-E; EXPRESSION; DISEASE; OBESE; RATS; HEPATOTOXICITY;
D O I
10.1097/MEG.0b013e328336000d
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background/aims This study examines the modifying effects of thymoquinone (TQ), an agent with antioxidant and hypolipidemic properties, on reactive oxygen species, antioxidant activity, and steatosis in livers of hyperlipidemic rabbit. Methods Oxidative stress was induced in New Zealand White rabbit by a high-cholesterol diet for 8 weeks. Four groups of six animals each were divided into a control group; a high cholesterol group (received 1% cholesterol diet); a high cholesterol/low TQ group (received 1% cholesterol diet plus TQ 10 mg/kg/day), and a high cholesterol/high TQ (20 mg/kg/day) group. Blood samples were taken at the end of the study and examined for fasting serum glucose, insulin, and aminotransaminases. Hepatic tissue samples were examined for malondialdehyde, protein carbonyls, antioxidant enzymes, and steatosis. Results There was severe hepatic steatosis and elevated serum alanine aminotransferase in the high cholesterol group (group II) but not in the high cholesterol and low or high TQ groups (groups III, IV). The hepatic reactive oxygen species activity in the high cholesterol group was significantly higher while the antioxidant enzymes were lower (P < 0.05) when compared with the control (group I) or either of the high cholesterol and TQ groups. Conclusion TQ attenuates hepatic oxidative stress in fatty liver injury induced by high-cholesterol diet in rabbits. Eur J Gastroenterol Hepatol 22:826-834 (C) 2010 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
引用
收藏
页码:826 / 834
页数:9
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