Purification and characterization of angiotensin converting enzyme-inhibitory peptides derived from Stichopus horrens: Stability study against the ACE and inhibition kinetics

被引：80

作者：

Forghani, Bita ^{[1
,2
]}

Zarei, Mohammad ^{[1
,3
]}

Ebrahimpour, Afshin ^{[1
]}

Philip, Robin ^{[4
]}

Bakar, Jamilah ^{[5
]}

Hamid, Azizah Abdul ^{[1
]}

Saari, Nazamid ^{[1
]}

机构：

[1] Univ Putra Malaysia, Fac Food Sci & Technol, Dept Food Sci, Serdang 43400, Malaysia

[2] Res Inst Food Sci & Technol, Mashhad, Iran

[3] Islamic Azad Univ, Sanandaj Branch, Dept Food Sci & Technol, Coll Agr & Nat Resources, Sanandaj, Iran

[4] Agilent Technol, U Chu Liang Bldg,22-F Unit A,Rama 4 Rd, Bangkok 10500, Thailand

[5] Univ Putra Malaysia, Fac Food Sci & Technol, Dept Food Technol, Serdang 43400, Malaysia

来源：

JOURNAL OF FUNCTIONAL FOODS | 2016年 / 20卷

关键词：

ACE-inhibitory peptides; Hypertension; Medicinal sea cucumber; Mode of inhibition; Isoelectric focusing technique; In vivo ACE inhibition assay; SEA-CUCUMBER; PROTEIN HYDROLYSATE; TRITERPENE GLYCOSIDES; MUSCLE PROTEIN; IN-VITRO; IDENTIFICATION; HYDROLYZATE; GELATIN;

D O I：

10.1016/j.jff.2015.10.025

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Stichopus horrens is the most popular species of sea cucumber due to strong beliefs of its numerous medicinal properties. In this study, ACE-inhibitory peptides of S. horrens generated through enzymatic hydrolysis using Alcalase were isolated. Three peptides EVSQGRP, CRQNTLGHNTQTSIAQ and VSRHFASYAN were found to exhibit high inhibition potency with IC50 values of 0.05, 0.08 and 0.21 mM, respectively. It was found that the EVSQGRP, VSRHFASYAN and SAAVGSP exhibiting mixed inhibition patterns were susceptible to degradation by ACE as well, suggesting that the mixed-mode inhibition could be a result of new generated peptide fragments while CRQNTLGHNTQTSIAQ inhibited ACE in a non-competitive manner. In vivo ACE inhibition studies showed that 400 mg/kg of Alcalase-generated proteolysate stabilized the blood pressure in normotensive rats. These results suggest that the hydrolysed protein components of S. horrens possess bioactive peptides that can be exploited as functional food ingredients against hypertension. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：276 / 290

页数：15

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