KT2 and RT2 modified antimicrobial peptides derived from Crocodylus siamensis Leucrocin I show activity against human colon cancer HCT-116 cells

被引:24
作者
Maijaroen, Surachai [1 ,2 ]
Jangpromma, Nisachon [2 ,3 ]
Daduang, Jureerut [4 ]
Klaynongsruang, Sompong [1 ,2 ]
机构
[1] Khon Kaen Univ, Dept Biochem, Fac Sci, Khon Kaen 40002, Thailand
[2] Khon Kaen Univ, Prot & Prote Res Ctr Commercial & Ind Purposes Pr, Fac Sci, Khon Kaen 40002, Thailand
[3] Khon Kaen Univ, Dept Integrated Sci, Forens Sci Program, Fac Sci, Khon Kaen 40002, Thailand
[4] Khon Kaen Univ, Fac Associated Med Sci, Ctr Res & Dev Med Diagnost Labs, Khon Kaen 40002, Thailand
关键词
Anticancer activity; Apoptosis; Colorectal cancer; Crocodile; Peptide; COLORECTAL-CANCER; ANTICANCER ACTIVITY; MEDIATED APOPTOSIS; CERVICAL-CANCER; DOWN-REGULATION; BREAST-CANCER; INDUCTION; RESISTANT; MIGRATION; PATHWAY;
D O I
10.1016/j.etap.2018.07.007
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Conventional colon cancer treatments have been associated with side effects. Consequently, the discovery of novel effective and safe therapies is urgently needed. Hence, cationic antimicrobial peptides KT2 and RT2 were evaluated towards human colon cancer HCT-116 cells. The MTT assay indicated that both KT2 and RT2 exhibited anticancer activity with good therapeutic indices, and were found to be non-toxic to non-cancerous Vero cells. The IC50 values of KT2 were determined as 111.96 and 90.25 mu g/mL while RT2 showed IC50 as 104.07 and 87.84 mu g/mL after 12 and 24 h treatments, respectively. Moreover, KT2 and RT2 treatment caused a significant reduction in PI3K, AKT1 and mTOR mRNA expression levels, which resulted in suppression either of HCT-116 proliferation or migration. The mechanism involved in apoptosis induction were due to decreased Bcl-2 and XIAP and increased p53, cytochrome c, caspase-2, caspase-3, caspase-8, and caspase-9 mRNA expression levels. These effects increased the level of cell cycle associated gene p21 and decreased cyclin B1 and cyclin D1 expression.
引用
收藏
页码:164 / 176
页数:13
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