Off-Column Amperometric Detection for Pressurized Capillary Electrochromatography

被引：3
作者：
Liu, Shaofeng [1 ,2 ]
Lin, Xucong [1 ]
Zhang, Xue [1 ]
Lue, Haixia [1 ]
Xie, Zenghong [1 ,3 ]
机构：
[1] Fuzhou Univ, Inst Food Safety & Environm Monitoring, Fuzhou 350108, Peoples R China
[2] CNTC, Zhengzhou Tobacco Res Inst, Dept Chem, Zhengzhou 450001, Peoples R China
[3] Xiamen Huaxia Vocat Coll, Xiamen 361024, Peoples R China
来源：
CHROMATOGRAPHIA | 2010年 / 71卷 / 7-8期
基金：
中国国家自然科学基金;
关键词：
Capillary electrochromatography; Off-column; Amperometric detection; MAGNETIC-RESONANCE-SPECTROSCOPY; MONOLITHIC STATIONARY PHASES; STEARYL-ACRYLATE MONOLITHS; CHEMILUMINESCENCE DETECTION; ELECTROCHEMICAL DETECTION; ZONE ELECTROPHORESIS; MASS-SPECTROMETRY; ETCHED JOINT; SEPARATION; ESTROGENS;
D O I：
10.1365/s10337-010-1529-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A system enabling coupling of pressurized capillary electrochromatography (pCEC) with off-column amperometric detection (AD) is reported in which conduction of the current in pCEC was achieved through a cellulose acetate-coated porous polymer joint, and the effect of the high-voltage field applied to pCEC for AD was also eliminated. Effects of supplementary pressure on the porous polymer joint and the effects on AD of capillary columns of different i.d. were investigated. The performance of the pCEC-AD system with the porous polymer joint was evaluated with phenol and hydroquinone using sulfonated stearyl methacrylate monolithic columns. The separation efficiency of the column in pCEC-AD, using the proposed off-column detection with the cellulose acetate membrane joint, was comparable with that of pCEC-UV using on-column detection. Compared with end-column detection using a 50 mu m i.d. capillary column without a joint, a higher signal-to-noise ratio was achieved, even using a 100 mu m i.d. capillary column with a joint. Successful separation and detection of dopamine and epinephrine were also achieved by use of this system.
引用
收藏
页码：659 / 665
页数：7
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