Improved poly-γ-glutamic acid production by chromosomal integration of the Vitreoscilla hemoglobin gene (vgb) in Bacillus subtilis

被引：70

作者：

Su, Yishan ^{[1
]}

Li, Xia ^{[1
]}

Liu, Qingzhi ^{[1
]}

Hou, Zhongwen ^{[1
]}

Zhu, Xiqiang ^{[1
]}

Guo, Xueping ^{[1
]}

Ling, Peixue ^{[1
]}

机构：

[1] Shandong Acad Pharmaceut Sci, Jinan 250101, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2010年 / 101卷 / 12期

关键词：

Vitreoscilla hemoglobin; Vitreoscilla hemoglobin gene; Chromosomal integration; Bacillus subtilis; gamma-PGA; ENHANCES GROWTH; EXPRESSION; PROTEINS;

D O I：

10.1016/j.biortech.2010.01.128

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

In order to alleviate oxygen limitation and improve the yield of poly-gamma-glutamic acid (gamma-PGA) during fermentation, the Vitreoscilla hemoglobin gene (vgb) was integrated into the chromosome of Bacillus subtilis and expressed during gamma-PGA production. The activity of the expressed Vitreoscilla hemoglobin (VHb) was confirmed by CO-difference spectrum. Expression of VHb enhanced cell growth under high viscosity fermentation conditions 1.26-fold and increased the yield of gamma-PGA 2.07-fold. These results indicate that the expression of VHb could be advantageous in high viscosity fermentation media. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：4733 / 4736

页数：4

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