CYCLINg through transcription Posttranslational modifications of P-TEFb regulate transcription elongation

被引:53
作者
Cho, Sungyoo [1 ]
Schroeder, Sebastian [1 ]
Ott, Melanie [1 ]
机构
[1] Univ Calif San Francisco, Gladstone Inst Virol & Immunol, San Francisco, CA 94143 USA
关键词
transcription elongation; RNA polymerase II; cyclin T; CDK9; RNA-POLYMERASE-II; BROMODOMAIN PROTEIN BRD4; DEPENDENT KINASE REGULATION; VIRUS TYPE-1 TRANSCRIPTION; 7SK SNRNA; HIV-1; TAT; CELL-CYCLE; T-LOOP; GENE-EXPRESSION; CDK9/CYCLIN T1;
D O I
10.4161/cc.9.9.11346
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The cyclin T/CDK9 complex, also called positive transcription elongation factor b (P-TEFb) phosphorylates the C-terminal domain of the large fragment of the RNA polymerase II. This action is a hallmark of the transition from transcription initiation to elongation. P-TEFb is itself modified by phosphorylation and ubiquitination. Recently, the core components of P-TEFb, cyclin T1 and CDK9, were identified as novel substrates of histone acetyltransferases. Here, we review how posttranslational modifications regulate the activity of the P-TEFb complex and discuss how acetylation of the complex optimizes transcription elongation in the context of other posttranslational modifications.
引用
收藏
页码:1697 / 1705
页数:9
相关论文
共 89 条
[1]   High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in vivo:: roles in promoter proximal pausing and transcription elongation [J].
Andrulis, ED ;
Guzmán, E ;
Döring, P ;
Werner, J ;
Lis, JT .
GENES & DEVELOPMENT, 2000, 14 (20) :2635-2649
[2]   Interplay between 7SK snRNA and oppositely charged regions in HEXIM1 direct the inhibition of P-TEFb [J].
Barboric, M ;
Kohoutek, J ;
Price, JP ;
Blazek, D ;
Price, DH ;
Peterlin, BM .
EMBO JOURNAL, 2005, 24 (24) :4291-4303
[3]   Ubiquitylation of Cdk9 by Skp2 facilitates optimal tat transactivation [J].
Barboric, M ;
Zhang, F ;
Besenicar, M ;
Plemenitas, A ;
Peterlin, BM .
JOURNAL OF VIROLOGY, 2005, 79 (17) :11135-11141
[4]   Tat competes with HEXIM1 to increase the active pool of P-TEFb for HIV-1 transcription [J].
Barboric, Matjaz ;
Yik, Jasper H. N. ;
Czudnochowski, Nadine ;
Yang, Zhiyuan ;
Chen, Ruichuan ;
Contreras, Xavier ;
Geyer, Matthias ;
Peterlin, B. Matija ;
Zhou, Qiang .
NUCLEIC ACIDS RESEARCH, 2007, 35 (06) :2003-2012
[5]   Chromatin poises miRNA- and protein-coding genes for expression [J].
Barski, Artem ;
Jothi, Raja ;
Cuddapah, Suresh ;
Cui, Kairong ;
Roh, Tae-Young ;
Schones, Dustin E. ;
Zhao, Keji .
GENOME RESEARCH, 2009, 19 (10) :1742-1751
[6]   The structure of P-TEFb (CDK9/cyclin T1), its complex with flavopiridol and regulation by phosphorylation [J].
Baumli, Sonja ;
Lolli, Graziano ;
Lowe, Edward D. ;
Troiani, Sonia ;
Rusconi, Luisa ;
Bullock, Alex N. ;
Debreczeni, Judit E. ;
Knapp, Stefan ;
Johnson, Louise N. .
EMBO JOURNAL, 2008, 27 (13) :1907-1918
[7]   Conserved P-TEFb-interacting domain of BRD4 inhibits HIV transcription [J].
Bisgrovet, Dwayne A. ;
Mahmoudi, Tokameh ;
Henklein, Peter ;
Verdin, Eric .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (34) :13690-13695
[8]   Oligomerization of HEXIM1 via 7SK snRNA and coiled-coil region directs the inhibition of P-TEFb [J].
Blazek, D ;
Barboric, M ;
Kohoutek, J ;
Oven, I ;
Peterlin, BM .
NUCLEIC ACIDS RESEARCH, 2005, 33 (22) :7000-7010
[9]   The multi-tasking P-TEFb complex [J].
Bres, Vanessa ;
Yoh, Sunnie M. ;
Jones, Katherine A. .
CURRENT OPINION IN CELL BIOLOGY, 2008, 20 (03) :334-340
[10]   Progression through the RNA Polymerase II CTD Cycle [J].
Buratowski, Stephen .
MOLECULAR CELL, 2009, 36 (04) :541-546