In vitro assessment of metabolic profile of Enterococcus strains of human origin

被引：25

作者：

Bhagwat, Ashlesha ^{[1
]}

Annapure, Uday S. ^{[1
]}

机构：

[1] Inst Chem Technol, Dept Food Engn & Technol, Mumbai 400019, Maharashtra, India

来源：

JOURNAL OF GENETIC ENGINEERING AND BIOTECHNOLOGY | 2019年 / 17卷 / 01期

关键词：

Enterococcus; Enzymes; BA; BSH; CLA; Antioxidant;

D O I：

10.1186/s43141-019-0009-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background In the present study, previously isolated, safe, and avirulent enterococci strains were exploited for their metabolic profile (Bhagwat et al., Asian J Pharm Clin Res 12: 2019). Results Thirteen enterococci strains of human origin produced important enzymes like amylase (0.5-0.7 mg ml(-1)), protease (192-264 mg ml(-1)), lipase (8-10 mg ml(-1)), bile salt hydrolase, conjugated linoleic acid (CLA), and lactic acid (highest 12 mg ml(-1)), thus implicating potential attributes of starter cultures in food and dairy industry. Biogenic amines like arginine and tryptamine were produced after 4 days above 25 degrees C. Castor oil (highest yield 60 mu g ml(-1)) and sunflower oil (highest yield 48 mu g ml(-1)) both proved to be excellent sources of CLA production. Reduction assays using FRAP, ABTS (above 83%), and DPPH (30-50%) revealed excellent radical scavenging properties of cell-free supernatants of Enterococcus strains. Conclusion The results implicate the future potential of application enterococci for therapeutic purpose as well as the food industry.

引用

页数：11

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