Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes

被引：25

作者：

Thompson, Nancy L. ^{[1
]}

Wang, Xiang ^{[1
]}

Navaratnarajah, Punya ^{[2
]}

机构：

[1] Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA

[2] Univ N Carolina, Dept Biochem & Biophys, Chapel Hill, NC 27599 USA

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2009年 / 168卷 / 01期

关键词：

Fluorescence microscopy; Optical microscopy; Evanescent; Fluctuation; Single molecule; Surface binding; Diffusion; Supported membranes; Cell membranes; CROSS-CORRELATION SPECTROSCOPY; SINGLE-MOLECULE ANALYSIS; EVANESCENT-WAVE; 2-PHOTON EXCITATION; IMAGE CORRELATION; FLUCTUATION SPECTROSCOPY; STATISTICAL ACCURACY; STANDARD-DEVIATION; PLASMA-MEMBRANE; 100; NM;

D O I：

10.1016/j.jsb.2009.02.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper, the conceptual basis and experimental design of total internal reflection with fluorescence correlation spectroscopy (TIR-FCS) is described. The few applications to date of TIR-FCS to supported membranes are discussed, in addition to a variety of applications not directly involving supported membranes. Methods related, but not technically equivalent, to TIR-FCS are also summarized. Future directions for TIR-FCS are outlined. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：95 / 106

页数：12

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