Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes

被引：25
|
作者：
Thompson, Nancy L. [1 ]
Wang, Xiang [1 ]
Navaratnarajah, Punya [2 ]
机构：
[1] Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA
[2] Univ N Carolina, Dept Biochem & Biophys, Chapel Hill, NC 27599 USA
来源：
JOURNAL OF STRUCTURAL BIOLOGY | 2009年 / 168卷 / 01期
关键词：
Fluorescence microscopy; Optical microscopy; Evanescent; Fluctuation; Single molecule; Surface binding; Diffusion; Supported membranes; Cell membranes; CROSS-CORRELATION SPECTROSCOPY; SINGLE-MOLECULE ANALYSIS; EVANESCENT-WAVE; 2-PHOTON EXCITATION; IMAGE CORRELATION; FLUCTUATION SPECTROSCOPY; STATISTICAL ACCURACY; STANDARD-DEVIATION; PLASMA-MEMBRANE; 100; NM;
D O I：
10.1016/j.jsb.2009.02.013
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this paper, the conceptual basis and experimental design of total internal reflection with fluorescence correlation spectroscopy (TIR-FCS) is described. The few applications to date of TIR-FCS to supported membranes are discussed, in addition to a variety of applications not directly involving supported membranes. Methods related, but not technically equivalent, to TIR-FCS are also summarized. Future directions for TIR-FCS are outlined. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码：95 / 106
页数：12
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