Application of TALEs, CRISPR/Cas and sRNAs as trans-acting regulators in prokaryotes

被引：27

作者：

Copeland, Matthew F. ^{[1
]}

Politz, Mark C. ^{[1
]}

Pfleger, Brian F. ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Chem & Biol Engn, Madison, WI 53706 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2014年 / 29卷

基金：

美国国家科学基金会;

关键词：

SEQUENCE-SPECIFIC CONTROL; DNA-BINDING SPECIFICITY; ARTIFICIAL SMALL RNAS; ESCHERICHIA-COLI; GENE-EXPRESSION; EFFECTORS; RECOGNITION; ACTIVATION; BACTERIA; SYSTEMS;

D O I：

10.1016/j.copbio.2014.02.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The last several years have witnessed an explosion in the understanding and use of novel, versatile trans-acting elements. TALEs, CRISPR/Cas, and sRNAs can be easily fashioned to bind any specific sequence of DNA (TALEs, CRISPR/Cas) or RNA (sRNAs) because of the simple rules governing their interactions with nucleic acids. This unique property enables these tools to repress the expression of genes at the transcriptional or post-transcriptional levels, respectively, without prior manipulation of cis-acting and/or chromosomal target DNA sequences. These tools are now being harnessed by synthetic biologists, particularly those in the eukaryotic community, for genome-wide regulation, editing, or epigenetic studies. Here we discuss the exciting opportunities for using TALEs, CRISPR/Cas, and sRNAs as synthetic trans-acting regulators in prokaryotes.

引用

页码：46 / 54

页数：9

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