Water-Soluble Isatoic Anhydrides: A Platform for RNA-SHAPE Analysis and Protein Bioconjugation

被引：8

作者：

Fessler, Adam B. ^{[1
]}

Dey, Abhishek ^{[2
]}

Garmon, Corey B. ^{[1
]}

Finis, Dominic S. ^{[1
]}

Saleh, Nemah-Allah ^{[1
]}

Fowler, Anthony J. ^{[3
]}

Jones, Daniel S. ^{[1
]}

Chakrabarti, Kausik ^{[2
]}

Ogle, Craig A. ^{[1
]}

机构：

[1] Univ N Carolina, Dept Chem, Charlotte, NC 28223 USA

[2] Univ N Carolina, Dept Biol Sci, Charlotte, NC 28223 USA

[3] NuChemie LLC, Charlotte, NC 28227 USA

来源：

BIOCONJUGATE CHEMISTRY | 2018年 / 29卷 / 09期

基金：

美国国家科学基金会;

关键词：

IN-VITRO; CELLS; NANOPARTICLES; CHEMISTRY; SECONDARY; BINDING;

D O I：

10.1021/acs.bioconjchem.8b00518

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

N-(3-Iodopropyl)isatoic anhydride (IPIA) has been demonstrated to serve as an efficient substrate for the development of an extended bioconjugation platform. Derivatives of IPIA are water-soluble and adaptable and share a common chromophore, rendering them easily quantifiable. We demonstrate the preparation of the readily diversified bioconjugation platform technology and application of the reagents in RNA-SHAPE analysis.

引用

页码：3196 / 3202

页数：7

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