Impact of uranium exposure on marine yeast, Yarrowia lipolytica: Insights into the yeast strategies to withstand uranium stress
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Kolhe, Nilesh
[1
,2
]
Zinjarde, Smita
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Savitribai Phule Pune Univ, Inst Bioinformat & Biotechnol, Pune 411007, Maharashtra, India
Savitribai Phule Pune Univ, Dept Microbiol, Pune 411007, Maharashtra, IndiaSavitribai Phule Pune Univ, Inst Bioinformat & Biotechnol, Pune 411007, Maharashtra, India
Zinjarde, Smita
[1
,3
]
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Acharya, Celin
[2
,4
]
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[1] Savitribai Phule Pune Univ, Inst Bioinformat & Biotechnol, Pune 411007, Maharashtra, India
[2] Bhabha Atom Res Ctr, Div Mol Biol, Mumbai 400085, Maharashtra, India
[3] Savitribai Phule Pune Univ, Dept Microbiol, Pune 411007, Maharashtra, India
[4] Homi Bhabha Natl Inst, Mumbai 400094, Maharashtra, India
A marine yeast, Yarrowia lipolytica, was evaluated for morphological, physiological and biochemical responses towards uranium (U) exposure at pH 7.5. The yeast revealed biphasic U binding - a rapid biosorption resulting in similar to 35% U binding within 15-30 min followed by a slow biomineralization process, binding up to similar to 45.5% U by 24 h on exposure to 50 mu M of uranyl carbonate. Scanning electron microscopy coupled with Energy Dispersive X-ray spectroscopy analysis of 24 h U challenged cells revealed the deposition of uranyl precipitates due to biomineralization. The loss of intracellular structures together with surface and subcellular localization of uranyl precipitates in 24 h U exposed cells were visualized by transmission electron microscopy. Cells treated with 50 mu M U exhibited membrane permeabilization which was higher at 200 mu M U. Enhanced reactive oxygen species (ROS) accumulation and lipid peroxidation, transient RNA degradation and protein oxidation were observed in U exposed cells. High superoxide dismutase levels coupled with uranium binding and bioprecipitation possibly helped in counteracting U stress in 50 mu M U treated cells. Resistance to U toxicity apparently developed under prolonged uranyl (50 mu M) incubations. However, cells could not cope up with toxicity at 200 mu M U due to impairment of resistance mechanisms.
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Bai, Jing
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Yin, Xiaojie
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Univ Chinese Acad Sci, Beijing, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Yin, Xiaojie
;
Zhu, Yongfeng
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Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou, Gansu, Peoples R China
Univ Chinese Acad Sci, Beijing, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Zhu, Yongfeng
;
Fan, Fangli
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Fan, Fangli
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Wu, Xiaolei
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Wu, Xiaolei
;
Tian, Wei
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Univ Chinese Acad Sci, Beijing, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Tian, Wei
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Tan, Cunmin
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Univ Chinese Acad Sci, Beijing, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Tan, Cunmin
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Zhang, Xin
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Univ Chinese Acad Sci, Beijing, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Zhang, Xin
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Wang, Yang
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Cao, Shiwei
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Univ Chinese Acad Sci, Beijing, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Cao, Shiwei
;
Fan, Fuyou
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Univ Chinese Acad Sci, Beijing, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Fan, Fuyou
;
Qin, Zhi
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Qin, Zhi
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Guo, Junsheng
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
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Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Bai, Jing
;
Yao, Huijun
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Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Yao, Huijun
;
Fan, Fangli
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Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Fan, Fangli
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Lin, Maosheng
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Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Lin, Maosheng
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Zhang, Lina
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Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Zhang, Lina
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Ding, Huajie
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Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Ding, Huajie
;
Lei, Fuan
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Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Lei, Fuan
;
Wu, Xiaolei
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Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Wu, Xiaolei
;
Li, Xiaofei
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Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Li, Xiaofei
;
Guo, Junsheng
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Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Guo, Junsheng
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Qin, Zhi
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Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
机构:
Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Bai, Jing
;
Yin, Xiaojie
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Univ Chinese Acad Sci, Beijing, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Yin, Xiaojie
;
Zhu, Yongfeng
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Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou, Gansu, Peoples R China
Univ Chinese Acad Sci, Beijing, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Zhu, Yongfeng
;
Fan, Fangli
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Fan, Fangli
;
Wu, Xiaolei
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Wu, Xiaolei
;
Tian, Wei
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Univ Chinese Acad Sci, Beijing, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Tian, Wei
;
Tan, Cunmin
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Univ Chinese Acad Sci, Beijing, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Tan, Cunmin
;
Zhang, Xin
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Univ Chinese Acad Sci, Beijing, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Zhang, Xin
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Wang, Yang
;
Cao, Shiwei
论文数: 0引用数: 0
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机构:
Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Univ Chinese Acad Sci, Beijing, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Cao, Shiwei
;
Fan, Fuyou
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Univ Chinese Acad Sci, Beijing, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Fan, Fuyou
;
Qin, Zhi
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
Qin, Zhi
;
Guo, Junsheng
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Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
机构:
Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Bai, Jing
;
Yao, Huijun
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Yao, Huijun
;
Fan, Fangli
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h-index: 0
机构:
Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Fan, Fangli
;
Lin, Maosheng
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Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Lin, Maosheng
;
Zhang, Lina
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h-index: 0
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Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Zhang, Lina
;
Ding, Huajie
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Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Ding, Huajie
;
Lei, Fuan
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Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Lei, Fuan
;
Wu, Xiaolei
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Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Wu, Xiaolei
;
Li, Xiaofei
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机构:
Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Li, Xiaofei
;
Guo, Junsheng
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h-index: 0
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Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
Guo, Junsheng
;
Qin, Zhi
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Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China