Amino acid phosphoramidate nucleotides as alternative substrates for HIV-1 reverse transcriptase

被引：59

作者：

Adelfinskaya, Olga ^{[1
]}

Herdewijn, Piet ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Rega Inst Med Res, Med Chem Lab, B-3000 Louvain, Belgium

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 23期

关键词：

amino acids; chain elongation; enzymes; pyrophosphate mimetics; reverse transcriptase;

D O I：

10.1002/anie.200605016

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Pick and choose: Amino acid phosphoramidites, in particular Asp-dAMP (dAMP = 2′-deoxyadenosine-5′-monophosphate) and His-dAMP, act in a template-dependent DNA synthesis assay as alternative substrates for reverse transcriptase. L-Aspartic acid can function as a leaving group during DNA synthesis and can be considered as a pyrophosphate mimetic in this reaction. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：4356 / 4358

页数：3

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