Influence of the collection and oxygenation method on quantitative bacterial composition in bronchoalveolar lavage fluid samples from healthy dogs

The purpose of the study was to evaluate the effects on quantitative and qualitative microbial content of endoscopic bronchoalveolar lavage fluid (BALF) in healthy dogs using a laryngeal mask airway (LMA). It was hypothesised that oropharyngeal protection might prevent contamination of BALF with oropharyngeal microflora. Ten healthy Beagle dogs were randomly assigned to two groups and underwent endoscopic BAL on two occasions, either via an open unprotected oropharynx with oxygen supply provided via a nasal catheter (NT) or through a sterile LMA. For the second sampling, groups were switched. BALF analysis included quantitative microbial culture, nucleated cell counts and cytology. The mean (+/- SD) number of colony forming units (CFU)/mL found in the BALF using the LMA was 25,610 +/- 22,943 in the right lung (RL) and 22,510 +/- 18,779 in the left (LL). With the NT technique, the figures were 21,068 +/- 19,375 for the RL and 16,060 +/- 15,523 for the LL, respectively. Nucleated cell counts/mu L were 691.0 +/- 181.6 (RL) and 734.0 +/- 171.6 (LL) for LMA, and 772.0 +/- 251.0 (RL) and 748 +/- 163.2 (LL) for NT. No significant differences were detected either in the number of CFU/mL or in the diversity of bacterial species with the two methods. A significant increase in BALF bacterial counts (with reduced species diversity) was observed on the second compared to the first sampling regardless of the method used. Protection of the oral cavity and oropharynx using an LMA had no significant influence on BALF bacterial counts. The findings suggest that with careful endoscope insertion, the risk of contamination of BALF by resident and transient oropharyngeal microflora can be negligible. (C) 2009 Elsevier Ltd. All rights reserved.