Nonspecific protection of heat-inactivated Mycobacterium bovis against Salmonella Choleraesuis infection in pigs

被引:15
作者
Vaz-Rodrigues, Rita [1 ]
Ferreras-Colino, Elisa [1 ]
Ugarte-Ruiz, Maria [2 ]
Pesciaroli, Michele [3 ]
Thomas, Jobin [4 ,5 ]
Garcia-Seco, Teresa [2 ]
Sevilla, Iker A. [6 ]
Perez-Sancho, Marta [2 ,7 ]
Mateo, Rafael [1 ]
Dominguez, Lucas [2 ,7 ]
Gortazar, Christian [1 ]
Risalde, Maria A. [8 ,9 ,10 ]
机构
[1] UCLM, SaBio Hlth & Biotechnol, Inst Invest Recursos Cineget IREC, CSIC, Ciudad Real, Spain
[2] Univ Complutense Madrid, VISAVET Hlth Surveillance Ctr, Madrid, Spain
[3] Ist Zooprofilatt Sperimentale Lombardia & Emilia, Brescia, Italy
[4] Indian Council Agr Res ICAR, New Delhi, India
[5] Anim Husb Dept, Thiruvananthapuram, Kerala, India
[6] NEIKER Inst Vasco Invest & Desarrollo Agrario, Anim Hlth Dept, Bizkaia, Spain
[7] Univ Complutense Madrid, Dept Anim Hlth, Fac Vet, Madrid, Spain
[8] Univ Cordoba UCO, Fac Vet, Dept Anat & Anat Patol Comparadas & Toxicol, Grp Invest Sanidad Anim & Zoonosis GISAZ, Cordoba, Spain
[9] Reina Sofia Univ Hosp, Maimonides Biomed Res Inst Cordoba IMIBIC, Infect Dis Unit, Clin Virol & Zoonosis Grp, Cordoba, Spain
[10] Inst Salud Carlos III, ISCIII CIBER Enfermedades Infecciosas, CIBERINFEC, Cordoba, Spain
关键词
Heat-inactivated Mycobacterium bovis; pig; Salmonella Choleraesuis; trained immunity; ORAL VACCINATION; IMMUNE-RESPONSES; TRAINED IMMUNITY; RED DEER; BCG; ENTERICA; EXPRESSION; CHALLENGE; STRAIN; MEMORY;
D O I
10.1186/s13567-022-01047-8
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Trained immunity is the capacity of innate immune cells to produce an improved response against a secondary infection after a previous unrelated infection. Salmonellosis represents a public health issue and affects the pig farming industry. In general, vaccination against salmonellosis is still facing problems regarding the control of distinct serovars. Therefore, we hypothesized that an immunostimulant based on heat inactivated Mycobacterium bovis (HIMB) could have an immune training effect in pigs challenged with Salmonella enterica serovar Choleraesuis (S. Choleraesuis) and decided to explore the amplitude of this non-specific immune response. For this purpose, twenty-four 10 days-old female piglets were randomly separated in three groups: immunized group (n = 10) received orally two doses of HIMB prior to the intratracheal S. Choleraesuis-challenge, positive control group (n = 9) that was only challenged with S. Choleraesuis, and negative control group (n = 5) that was neither immunized nor infected. All individuals were necropsied 21 days post-challenge. HIMB improved weight gain and reduced respiratory symptoms and pulmonary lesions caused by S. Choleraesuis in pigs. Pigs immunized with HIMB showed higher cytokine production, especially of serum TNF alpha and lung CCL28, an important mediator of mucosal trained immunity. Moreover, immunized pigs showed lower levels of the biomarker of lipid oxidation malondialdehyde and higher activity of the antioxidant enzyme superoxide dismutase than untreated challenged pigs. However, the excretion and tissue colonization of S. Choleraesuis remained unaffected. This proof-of-concept study suggests beneficial clinical, pathological, and heterologous immunological effects against bacterial pathogens within the concept of trained immunity, opening avenues for further research.
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页数:16
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