Recent trends in the analysis of vitamin D and its metabolites in milk - A review

被引:46
作者
Kasalova, Eva [1 ,2 ]
Aufartova, Jana [1 ,2 ]
Krcmova, Lenka Kujovska [1 ,2 ]
Solichova, Dagmar [2 ]
Solich, Petr [1 ]
机构
[1] Charles Univ Prague, Fac Pharm, Dept Analyt Chem, Hradec Kralove 50005, Czech Republic
[2] Univ Hosp, Internal Gerontometab Clin 3, Hradec Kralove 50005, Czech Republic
关键词
Vitamin D; Breast milk; Sample preparation; LC-MS; LC-UV; FAT-SOLUBLE VITAMINS; TANDEM MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; BREAST-MILK; DAIRY-PRODUCTS; INFANT; EXTRACTION; D-2; SUPPLEMENTATION; IDENTIFICATION;
D O I
10.1016/j.foodchem.2014.08.102
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Vitamin D plays an important role in calcium metabolism and affects other metabolic pathways. Despite the intense interest in vitamin D, no comprehensive overview addressing the analysis of vitamin D in milk has been published. Historically, immunoassay techniques have been mainly used for the routine quantification of vitamin D and its metabolites. However, the greater accuracy and precision of chromatography makes it one of the most important methods in the analysis of vitamin D. The determination of vitamin D and its metabolites by LC-MS is the gold standard for its assessment. LC-MS has unique advantages for vitamin D determination and quantification due to its high sensitivity and specificity. In this review, the current status of vitamin D and its metabolites analysis in milk, human and bovine, including sample pre-treatment and chromatography analysis, are critically discussed and summarised. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:177 / 190
页数:14
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