A new semi-solid agar dilution method for determining amoxycillin, clarithromycin and azithromycin MICs for Helicobacter pylori isolates

被引：14
作者：
Kobayashi, I
Hasegawa, M
Saika, T
Nishida, M
Fujioka, T
Nasu, M
机构：
[1] Mitsubishi Kagaku Bio Clin Labs, Chemotherapy Div, Itabashi Ku, Tokyo 174, Japan
[2] Oita Med Univ, Dept Internal Med 2, Oita 87955, Japan
来源：
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | 1997年 / 40卷 / 05期
关键词：
D O I：
10.1093/jac/40.5.713
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The MICs of amoxycillin, clarithromycin and azithromycin for 26 strains of Helicobacter pylori were determined using Mueller-Hinton semi-solid agar (SSA) without CO2 incubation, as in the conventional agar dilution method, The tested H. pylori strains grew satisfactorily in the SSA method within 48 h of incubation, Reasonable values of MICs of three antibiotics for these strains were obtained by this method, avoiding the inactivation of macrolides due to acidification of the medium. By this method, the MICs of the antibiotics for the reference strains were within the acceptable NCCLS ranges for quality control.
引用
收藏
页码：713 / 716
页数：4
相关论文
共 8 条
[1]   EFFECT OF AN ACIDIC ENVIRONMENT ON THE SUSCEPTIBILITY OF HELICOBACTER-PYLORI TO TROSPECTOMYCIN AND OTHER ANTIMICROBIAL AGENTS [J].
DEBETSOSSENKOPP, YJ ;
NAMAVAR, F ;
MACLAREN, DM .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1995, 14 (04) :353-355
[2]   EFFECT OF TREATMENT ON CAMPYLOBACTER-PYLORI IN PEPTIC DISEASE - A RANDOMIZED PROSPECTIVE TRIAL [J].
HUMPHREYS, H ;
BOURKE, S ;
DOOLEY, C ;
MCKENNA, D ;
POWER, B ;
KEANE, CT ;
SWEENEY, EC ;
OMORAIN, C .
GUT, 1988, 29 (03) :279-283
[3]   CAMPYLOBACTER LIKE ORGANISMS ON THE GASTRIC-MUCOSA - CULTURE, HISTOLOGICAL, AND SEROLOGICAL STUDIES [J].
JONES, DM ;
LESSELLS, AM ;
ELDRIDGE, J .
JOURNAL OF CLINICAL PATHOLOGY, 1984, 37 (09) :1002-1006
[4]   EFFECT OF PH VARIATION ON THE SUSCEPTIBILITY OF HELICOBACTER-PYLORI TO 3 MACROLIDE ANTIMICROBIAL AGENTS AND TEMAFLOXACIN [J].
MALANOSKI, GJ ;
ELIOPOULOS, GM ;
FERRARO, MJ ;
MOELLERING, RC .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1993, 12 (02) :131-133
[5]  
MARSHALL BJ, 1984, LANCET, V1, P1311
[6]   CAMPYLOBACTER PYLORIDIS AND ASSOCIATED GASTRITIS - INVESTIGATOR BLIND, PLACEBO CONTROLLED TRIAL OF BISMUTH SALICYLATE AND ERYTHROMYCIN ETHYLSUCCINATE [J].
MCNULTY, CAM ;
GEARTY, JC ;
CRUMP, B ;
DAVIS, M ;
DONOVAN, IA ;
MELIKIAN, V ;
LISTER, DM ;
WISE, R .
BRITISH MEDICAL JOURNAL, 1986, 293 (6548) :645-649
[7]  
*NAT COMM CLIN LAB, 1995, M7A3 NCCLS
[8]   CURE OF DUODENAL-ULCER ASSOCIATED WITH ERADICATION OF HELICOBACTER-PYLORI [J].
RAUWS, EAJ ;
TYTGAT, GNJ .
LANCET, 1990, 335 (8700) :1233-1235
1