Proteolytic Cleavage of Versican During Limb Joint Development

被引：10

作者：

Capehart, Anthony A. ^{[1
]}

机构：

[1] E Carolina Univ, Dept Biol, Greenville, NC 27858 USA

来源：

ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY | 2010年 / 293卷 / 02期

关键词：

versican; ECM; synovial joint development; limb development; joint interzone; FACTOR-LIKE MOTIFS; ARTICULAR-CARTILAGE; G1; DOMAIN; IN-VIVO; PG-M; SYNOVIAL JOINT; CELL-ADHESION; LINK PROTEIN; MOUSE LIMB; CHICK LIMB;

D O I：

10.1002/ar.21049

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Versican is highly expressed in developing joint interzones during limb morphogenesis. This study was undertaken to examine whether proteolytic cleavage of versican occurs that could potentially impact its function during the process of embryonic synovial joint formation. Using an antibody to the DPEAAE neoepitope generated by ADAMTS proteolysis, versican amino terminal cleavage fragments were detected in joint interzones at 12-16 days post coitum (dpe). ADAMTS-1 localization overlapped that of DPEAAE-reactive versican fragments suggesting it as one possible protease activity involved in processing of versican in the interzone. Results show that increased cleavage of versican in the interzone accompanies cavitation and suggests that proteolytic modification of versican may be important during the process of synovial joint maturation. Anat Rec, 293:208-214, 2010. (C) 2010 Wiley-Liss, Inc.

引用

页码：208 / 214

页数：7

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