Multiplex genotyping of PCR products with MassTag-labeled primers

被引：74

作者：

Haff, LA

Smirnov, IP

机构：

[1] PerSeptive Biosystems, Inc., Framingham, MA 01701

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 18期

关键词：

D O I：

10.1093/nar/25.18.3749

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple mass spectrometric based assay, the pinpoint assay, has previously been described for typing single nucleotide polymorphisms. The identity of a polymorphism is determined by mass differences of single base extended genotyping primers as determined by MALDI-TOF mass spectrometry. A simple method for multiplexing the assay is described, employing multiple primers with 5' oligo(dT) sequences (MassTags) which serve to mass discriminate the peaks of multiple extended and non-extended primers. The assay is extremely rapid and requires no labeling reagents.

引用

页码：3749 / 3750

页数：2

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